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Cre-regulated Lentiviral RNAi Vectors
The laboratory of Tyler Jacks (MIT) has deposited the following lentiviral vectors that allow for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice.
| ID | Plasmid | Description |
|---|---|---|
| 11586 | pSico PGK puro | Addition of Cre causes the puromycin gene to be recombined out of the construct, activating shRNA expression. |
| 11578 | pSico | Addition of Cre causes EGFP to be recombined out of the construct, activating shRNA expression. |
| 12084 | pSicoR PGK puro | Addition of Cre causes both puromycin and shRNA to be recombined out of the construct, turning OFF shRNA expression. |
| 11579 | pSicoR | Addition of Cre causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression. |
More information:
- pSico protocols from the Tyler Jacks laboratory at MIT.
- See Packaging Plasmids that can be used with these vectors.
- A review of conditional systems for RNA interference (Nature Methods 2006 Aug 23).
- Ventura et. al. 2004. Cre-lox-regulated conditional RNA interference from transgenes. PNAS 2004 Jul 13;101(28):10380-5. (PubMed)