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Tet-regulated Lentiviral RNAi Vectors

The laboratories of Patrick Aebischer and Didier Trono (EPFL) have deposited a lentiviral vector-based system for drug-controllable expression of polymerase II promoter-controlled transgenes or polymerase III promoter-controlled sequences encoding shRNAs.

ID Plasmid PolII Promoter Transgene Tet PolIII Promoter
11643 pLVCT-tTR-KRAB CAG GFP Tet-on H1 from pLVTHM
11779 pLVCT-rtTR-KRAB-2SM2 CAG GFP Tet-off H1 from pLVTHM
11644 pLVET-tTR-KRAB hEF-1alpha GFP Tet-on H1 from pLVTHM
11648 pLVPRT-tTR-KRAB hPrion GFP Tet-on H1 from pLVTHM
11642 pLVPT-tTR-KRAB mPGK GFP Tet-on H1 from pLVTHM
11652 pLVPT-rtTR-KRAB-2SM2 mPGK GFP Tet-off H1 from pLVTHM
11651 pLVUT-tTR-KRAB hUbiquitin GFP Tet-on H1 from pLVTHM
11649 pLVUT-shp53-tTR-KRAB hUbiquitin GFP Tet-on H1 driving p53 shRNA

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