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Myristoylated Kinase Library Kit
(Kit # 1000000012 )

Depositing Labs:   William Hahn, Jean Zhao

This library consists of 190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF. Academic and non-profit laboratories may request these vectors from Addgene for research and educational use.

This kit consists of 2 plates, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

$650 USD + shipping
Available to Academic and Nonprofits Only

Original Publication

Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell. 2007 Jun 15;129(6):1065-79. PubMed.

Description

This kit includes only the kinase library clones from the Boehm, Zhao, Yao et al. Cell 2007 publication. All other plasmids from the publication can be found here.

Kit Documentation

Icon Myristoylated Kinase Library Kit Info (105.0 KB)

Links

This kit includes only the kinase library clones from the Boehm, Zhao, Yao et al. Cell 2007 publication. All other plasmids from the publication can be found here.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmids expressing myristoylated kinases were a gift from William Hahn and Jean Zhao (Addgene kit # 1000000012)"

For your Reference section:
Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell. 2007 Jun 15;129(6):1065-79. PubMed.

Resistance Color Key:

 
  Ampicillin  

Inventory

Well Plasmid Resistance
1 / A / 1 pWZL Neo Myr Flag CKS1B
  Ampicillin
1 / A / 2 pWZL Neo Myr Flag TK1
  Ampicillin
1 / A / 3 pWZL Neo Myr Flag PIM1
  Ampicillin
1 / A / 4 pWZL Neo Myr Flag CDK9
  Ampicillin
1 / A / 5 pWZL Neo Myr Flag CHEK1
  Ampicillin
1 / A / 6 pWZL Neo Myr Flag SGK
  Ampicillin
1 / A / 7 pWZL Neo Myr Flag DYRK2
  Ampicillin
1 / A / 8 pWZL Neo Myr Flag PLK1
  Ampicillin
1 / A / 9 pWZL Neo Myr Flag MAPK7
  Ampicillin
1 / A / 10 pWZL Neo Myr Flag AKT1
  Ampicillin
1 / A / 11 pWZL Neo Myr Flag UCK2
  Ampicillin
1 / A / 12 pWZL Neo Myr Flag CKS2
  Ampicillin
1 / B / 1 pWZL Neo Myr Flag STK17B
  Ampicillin
1 / B / 2 pWZL Neo Myr Flag CAMKV
  Ampicillin
1 / B / 3 pWZL Neo Myr Flag CLK3
  Ampicillin
1 / B / 4 pWZL Neo Myr Flag MPP1
  Ampicillin
1 / B / 5 pWZL Neo Myr Flag TTK
  Ampicillin
1 / B / 6 pWZL Neo Myr Flag PIK4CB
  Ampicillin
1 / B / 7 pWZL Neo Myr Flag PKN2
  Ampicillin
1 / B / 8 pWZL Neo Myr Flag CKM
  Ampicillin
1 / B / 9 pWZL Neo Myr Flag STK38L
  Ampicillin
1 / B / 10 pWZL Neo Myr Flag LCK
  Ampicillin
1 / B / 12 pWZL Neo Myr Flag MATK
  Ampicillin
1 / C / 1 pWZL Neo Myr Flag MAP2K5
  Ampicillin
1 / C / 2 pWZL Neo Myr Flag CDK5
  Ampicillin
1 / C / 3 pWZL Neo Myr Flag IKBKE
  Ampicillin
1 / C / 4 pWZL Neo Myr Flag PAPSS1
  Ampicillin
1 / C / 5 pWZL Neo Myr Flag CSNK1G2
  Ampicillin
1 / C / 6 pWZL Neo Myr Flag PCTK1
  Ampicillin
1 / C / 7 pWZL Neo Myr Flag DAK
  Ampicillin
1 / C / 8 pWZL Neo Myr Flag BMX
  Ampicillin
1 / C / 9 pWZL Neo Myr Flag TAOK3
  Ampicillin
1 / C / 10 pWZL Neo Myr Flag PRKCZ
  Ampicillin
1 / C / 11 pWZL Neo Myr Flag CALM2
  Ampicillin
1 / C / 12 pWZL Neo Myr Flag CDC2
  Ampicillin
1 / D / 1 pWZL Neo Myr Flag RIOK3
  Ampicillin
1 / D / 2 pWZL Neo Myr Flag GALK2
  Ampicillin
1 / D / 3 pWZL Neo Myr Flag PIP5K1A
  Ampicillin
1 / D / 4 pWZL Neo Myr Flag NEK6
  Ampicillin
1 / D / 5 pWZL Neo Myr Flag DGKG
  Ampicillin
1 / D / 6 pWZL Neo Myr Flag CDK2
  Ampicillin
1 / D / 7 pWZL Neo Myr Flag CDK7
  Ampicillin
1 / D / 8 pWZL Neo Myr Flag CKMT1A
  Ampicillin
1 / D / 9 pWZL Neo Myr Flag GCK
  Ampicillin
1 / D / 10 pWZL Neo Myr Flag CLK2
  Ampicillin
1 / D / 11 pWZL Neo Myr Flag RPS6KA5
  Ampicillin
1 / D / 12 pWZL Neo Myr Flag RPS6KA2
  Ampicillin
1 / E / 1 pWZL Neo Myr Flag RET
  Ampicillin
1 / E / 2 pWZL Neo Myr Flag FASTK
  Ampicillin
1 / E / 3 pWZL Neo Myr Flag CDK4
  Ampicillin
1 / E / 4 pWZL Neo Myr Flag MAPK14
  Ampicillin
1 / E / 5 pWZL Neo Myr Flag LIMK1
  Ampicillin
1 / E / 6 pWZL Neo Myr Flag BLK
  Ampicillin
1 / E / 7 pWZL Neo Myr Flag MAP3K7
  Ampicillin
1 / E / 8 pWZL Neo Myr Flag PKM2
  Ampicillin
1 / E / 9 pWZL Neo Myr Flag TYK2
  Ampicillin
1 / E / 10 pWZL Neo Myr Flag MKNK1
  Ampicillin
1 / E / 11 pWZL Neo Myr Flag PRKRA
  Ampicillin
1 / E / 12 pWZL Neo Myr Flag MAPK13
  Ampicillin
1 / F / 1 pWZL Neo Myr Flag AURKA
  Ampicillin
1 / F / 2 pWZL Neo Myr Flag PLAU
  Ampicillin
1 / F / 3 pWZL Neo Myr Flag PCTK3
  Ampicillin
1 / F / 4 pWZL Neo Myr Flag OXSR1
  Ampicillin
1 / F / 5 pWZL Neo Myr Flag MAP3K6
  Ampicillin
1 / F / 6 pWZL Neo Myr Flag RPS6KB2
  Ampicillin
1 / F / 7 pWZL Neo Myr Flag VRK2
  Ampicillin
1 / F / 8 pWZL Neo Myr Flag FGR
  Ampicillin
1 / F / 9 pWZL Neo Myr Flag RIOK2
  Ampicillin
1 / F / 10 pWZL Neo Myr Flag PCK2
  Ampicillin
1 / F / 11 pWZL Neo Myr Flag LIMK2
  Ampicillin
1 / F / 12 pWZL Neo Myr Flag ACVR1
  Ampicillin
1 / G / 1 pWZL Neo Myr Flag STK3
  Ampicillin
1 / G / 2 pWZL Neo Myr Flag AMHR2
  Ampicillin
1 / G / 3 pWZL Neo Myr Flag GK2
  Ampicillin
1 / G / 4 pWZL Neo Myr Flag ADCK5
  Ampicillin
1 / G / 5 pWZL Neo Myr Flag CSNK1A1L
  Ampicillin
1 / G / 6 pWZL Neo Myr Flag MAP2K7
  Ampicillin
1 / G / 7 pWZL Neo Myr Flag PIP5K3
  Ampicillin
1 / G / 8 pWZL Neo Myr Flag NEK11
  Ampicillin
1 / G / 9 pWZL Neo Myr Flag NEK3
  Ampicillin
1 / G / 10 pWZL Neo Myr Flag GRK6
  Ampicillin
1 / G / 11 pWZL Neo Myr Flag HIPK1
  Ampicillin
1 / G / 12 pWZL Neo Myr Flag PMVK
  Ampicillin
1 / H / 1 pWZL Neo Myr Flag PACSIN1
  Ampicillin
1 / H / 2 pWZL Neo Myr Flag ILK
  Ampicillin
1 / H / 3 pWZL Neo Myr Flag CLK1
  Ampicillin
1 / H / 4 pWZL Neo Myr Flag STK33
  Ampicillin
1 / H / 5 pWZL Neo Myr Flag SYK
  Ampicillin
1 / H / 6 pWZL Neo Myr Flag ADRBK1
  Ampicillin
1 / H / 7 pWZL Neo Myr Flag RPS6KL1
  Ampicillin
1 / H / 8 pWZL Neo Myr Flag HK2
  Ampicillin
1 / H / 9 pWZL Neo Myr Flag CERK
  Ampicillin
1 / H / 10 pWZL Neo Myr Flag PDXK
  Ampicillin
1 / H / 11 pWZL Neo Myr Flag ADRBK2
  Ampicillin
1 / H / 12 pWZL Neo Myr Flag PIK3R3
  Ampicillin
2 / A / 1 pWZL Neo Myr Flag PCTK2
  Ampicillin
2 / A / 2 pWZL Neo Myr Flag MAPKAP1
  Ampicillin
2 / A / 3 pWZL Neo Myr Flag ULK4
  Ampicillin
2 / A / 4 pWZL Neo Myr Flag ITK
  Ampicillin
2 / A / 5 pWZL Neo Myr Flag BTK
  Ampicillin
2 / A / 6 pWZL Neo Myr Flag RPSK6A3
  Ampicillin
2 / A / 7 pWZL Neo Myr Flag EPHA4
  Ampicillin
2 / A / 8 pWZL Neo Myr Flag MAP3K8
  Ampicillin
2 / A / 9 pWZL Neo Myr Flag AKT3
  Ampicillin
2 / A / 10 pWZL Neo Myr Flag CAMKK1
  Ampicillin
2 / A / 11 pWZL Neo Myr Flag GK
  Ampicillin
2 / A / 12 pWZL Neo Myr Flag YES1
  Ampicillin
2 / B / 2 pWZL Neo Myr Flag DLG5
  Ampicillin
2 / B / 3 pWZL Neo Myr Flag RPS6KA6
  Ampicillin
2 / B / 4 pWZL Neo Myr Flag CAMK4
  Ampicillin
2 / B / 5 pWZL Neo Myr Flag HCK
  Ampicillin
2 / B / 6 pWZL Neo Myr Flag RPS6KB1
  Ampicillin
2 / B / 7 pWZL Neo Myr Flag MAPK6
  Ampicillin
2 / B / 8 pWZL Neo Myr Flag TEC
  Ampicillin
2 / B / 9 pWZL Neo Myr Flag PRKCD
  Ampicillin
2 / B / 10 pWZL Neo Myr Flag PIK4CA
  Ampicillin
2 / B / 11 pWZL Neo Myr Flag PIK3CB
  Ampicillin
2 / B / 12 pWZL Neo Myr Flag VRK3
  Ampicillin
2 / C / 1 pWZL Neo Myr Flag PI4K2B
  Ampicillin
2 / C / 2 pWZL Neo Myr Flag FRK
  Ampicillin
2 / C / 3 pWZL Neo Myr Flag PRKAA1
  Ampicillin
2 / C / 4 pWZL Neo Myr Flag GRK5
  Ampicillin
2 / C / 5 pWZL Neo Myr Flag PLK2
  Ampicillin
2 / C / 6 pWZL Neo Myr Flag PFKM
  Ampicillin
2 / C / 7 pWZL Neo Myr Flag MOBKL2A
  Ampicillin
2 / C / 8 pWZL Neo Myr Flag AAK1
  Ampicillin
2 / C / 9 pWZL Neo Myr Flag TSSK1B
  Ampicillin
2 / C / 10 pWZL Neo Myr Flag MAST1
  Ampicillin
2 / C / 11 pWZL Neo Myr Flag PDIK1L
  Ampicillin
2 / C / 12 pWZL Neo Myr Flag NADK
  Ampicillin
2 / D / 1 pWZL Neo Myr Flag DYRK4
  Ampicillin
2 / D / 2 pWZL Neo Myr Flag MVK
  Ampicillin
2 / D / 3 pWZL Neo Myr Flag PAK4
  Ampicillin
2 / D / 4 pWZL Neo Myr Flag TESK1
  Ampicillin
2 / D / 5 pWZL Neo Myr Flag MAPK12
  Ampicillin
2 / D / 6 pWZL Neo Myr Flag ITPKB
  Ampicillin
2 / D / 7 pWZL Neo Myr Flag IHPK2
  Ampicillin
2 / D / 8 pWZL Neo Myr Flag SPHK2
  Ampicillin
2 / D / 9 pWZL Neo Myr Flag PIK3CG
  Ampicillin
2 / D / 10 pWZL Neo Myr Flag STK32C
  Ampicillin
2 / D / 11 pWZL Neo Myr Flag CKB
  Ampicillin
2 / D / 12 pWZL Neo Myr Flag ITPK1
  Ampicillin
2 / E / 1 pWZL Neo Myr Flag GAK
  Ampicillin
2 / E / 2 pWZL Neo Myr Flag PRKACG
  Ampicillin
2 / E / 3 pWZL Neo Myr Flag STK40
  Ampicillin
2 / E / 4 pWZL Neo Myr Flag PDPK1
  Ampicillin
2 / E / 5 pWZL Neo Myr Flag HK1
  Ampicillin
2 / E / 6 pWZL Neo Myr Flag PIK3R5
  Ampicillin
2 / E / 7 pWZL Neo Myr Flag PNKP
  Ampicillin
2 / E / 8 pWZL Neo Myr Flag PRKAG2
  Ampicillin
2 / E / 9 pWZL Neo Myr Flag NUAK2
  Ampicillin
2 / E / 10 pWZL Neo Myr Flag SNF1LK
  Ampicillin
2 / E / 11 pWZL Neo Myr Flag HK3
  Ampicillin
2 / E / 12 pWZL Neo Myr Flag PIP5K2A
  Ampicillin
2 / F / 1 pWZL Neo Myr Flag PIP5K1B
  Ampicillin
2 / F / 2 pWZL Neo Myr Flag PBK
  Ampicillin
2 / F / 3 pWZL Neo Myr Flag STK32B
  Ampicillin
2 / F / 4 pWZL Neo Myr Flag CMPK
  Ampicillin
2 / F / 5 pWZL Neo Myr Flag ADPGK
  Ampicillin
2 / F / 6 pWZL Neo Myr Flag MAP3K14
  Ampicillin
2 / F / 7 pWZL Neo Myr Flag CSNK1G1
  Ampicillin
2 / F / 8 pWZL Neo Myr Flag TNK2
  Ampicillin
2 / F / 9 pWZL Neo Myr Flag NTRK3
  Ampicillin
2 / F / 10 pWZL Neo Myr Flag DGUOK
  Ampicillin
2 / F / 11 pWZL Neo Myr Flag MOBKL1A
  Ampicillin
2 / F / 12 pWZL Neo Myr Flag NME7
  Ampicillin
2 / G / 1 pWZL Neo Myr Flag FGFR1
  Ampicillin
2 / G / 2 pWZL Neo Myr Flag PFKL
  Ampicillin
2 / G / 3 pWZL Neo Myr Flag PKN1
  Ampicillin
2 / G / 4 pWZL Neo Myr Flag PLK4
  Ampicillin
2 / G / 5 pWZL Neo Myr Flag CKMT2
  Ampicillin
2 / G / 6 pWZL Neo Myr Flag RIOK1
  Ampicillin
2 / G / 7 pWZL Neo Myr Flag STK32B
  Ampicillin
2 / G / 8 pWZL Neo Myr Flag ADCK4
  Ampicillin
2 / G / 9 pWZL Neo Myr Flag STK4
  Ampicillin
2 / G / 10 pWZL Neo Myr Flag TIE1
  Ampicillin
2 / G / 11 pWZL Neo Myr Flag PTK2
  Ampicillin
2 / G / 12 pWZL Neo Myr Flag SRPK2
  Ampicillin
2 / H / 1 pWZL Neo Myr Flag PRKACB
  Ampicillin
2 / H / 2 pWZL Neo Myr Flag MAP2K6
  Ampicillin
2 / H / 3 pWZL Neo Myr Flag PRKAR2A
  Ampicillin
2 / H / 4 pWZL Neo Myr Flag AXL
  Ampicillin
2 / H / 5 pWZL Neo Myr Flag PDK1
  Ampicillin
2 / H / 6 pWZL Neo Myr Flag CAMK1G
  Ampicillin
2 / H / 7 pWZL Neo Myr Flag TBK1
  Ampicillin
2 / H / 8 pWZL Neo Myr Flag STK32A
  Ampicillin
2 / H / 9 pWZL Neo Myr Flag TSSK6
  Ampicillin
2 / H / 10 pWZL Neo Myr Flag CSNK1E
  Ampicillin
2 / H / 11 pWZL Neo Myr Flag CAMK2D
  Ampicillin
2 / H / 12 pWZL Neo Myr Flag CAMK2B
  Ampicillin
Data from: CALCULATED @ 2014-11-24 10:19:40.490891