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Myristoylated Kinase Library Kit
(Kit # 1000000012 )

Depositing Labs:   William Hahn, Jean Zhao

This library consists of 190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF. Academic and nonprofit laboratories may request these vectors from Addgene for research and educational use.

This kit consists of 2 plates, and will be shipped as bacterial glycerol stocks, 96-well format, on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs.

Add to Cart
$650 USD + shipping

Available to academics and nonprofits only.

Original Publication

Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell . 2007 Jun 15;129(6):1065-79. PubMed .

Description

This kit includes only the kinase library clones from the Boehm, Zhao, Yao et al. Cell 2007 publication. All other plasmids from the publication can be found here .

Kit Documentation

Icon Myristoylated Kinase Library Kit Info (105.0 KB)

Links

This kit includes only the kinase library clones from the Boehm, Zhao, Yao et al. Cell 2007 publication. All other plasmids from the publication can be found here .

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:
"The plasmids expressing myristoylated kinases were a gift from William Hahn and Jean Zhao (Addgene kit # 1000000012)"

For your Reference section:
Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell . 2007 Jun 15;129(6):1065-79. PubMed .

Myristoylated Kinase Library - #1000000012

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin

Inventory

Well Plasmid Resistance
Plate 1 / A / 1 pWZL Neo Myr Flag CKS1B
Ampicillin
Plate 1 / A / 2 pWZL Neo Myr Flag TK1
Ampicillin
Plate 1 / A / 3 pWZL Neo Myr Flag PIM1
Ampicillin
Plate 1 / A / 4 pWZL Neo Myr Flag CDK9
Ampicillin
Plate 1 / A / 5 pWZL Neo Myr Flag CHEK1
Ampicillin
Plate 1 / A / 6 pWZL Neo Myr Flag SGK
Ampicillin
Plate 1 / A / 7 pWZL Neo Myr Flag DYRK2
Ampicillin
Plate 1 / A / 8 pWZL Neo Myr Flag PLK1
Ampicillin
Plate 1 / A / 9 pWZL Neo Myr Flag MAPK7
Ampicillin
Plate 1 / A / 10 pWZL Neo Myr Flag AKT1
Ampicillin
Plate 1 / A / 11 pWZL Neo Myr Flag UCK2
Ampicillin
Plate 1 / A / 12 pWZL Neo Myr Flag CKS2
Ampicillin
Plate 1 / B / 1 pWZL Neo Myr Flag STK17B
Ampicillin
Plate 1 / B / 2 pWZL Neo Myr Flag CAMKV
Ampicillin
Plate 1 / B / 3 pWZL Neo Myr Flag CLK3
Ampicillin
Plate 1 / B / 4 pWZL Neo Myr Flag MPP1
Ampicillin
Plate 1 / B / 5 pWZL Neo Myr Flag TTK
Ampicillin
Plate 1 / B / 6 pWZL Neo Myr Flag PIK4CB
Ampicillin
Plate 1 / B / 7 pWZL Neo Myr Flag PKN2
Ampicillin
Plate 1 / B / 8 pWZL Neo Myr Flag CKM
Ampicillin
Plate 1 / B / 9 pWZL Neo Myr Flag STK38L
Ampicillin
Plate 1 / B / 10 pWZL Neo Myr Flag LCK
Ampicillin
Plate 1 / B / 12 pWZL Neo Myr Flag MATK
Ampicillin
Plate 1 / C / 1 pWZL Neo Myr Flag MAP2K5
Ampicillin
Plate 1 / C / 2 pWZL Neo Myr Flag CDK5
Ampicillin
Plate 1 / C / 3 pWZL Neo Myr Flag IKBKE
Ampicillin
Plate 1 / C / 4 pWZL Neo Myr Flag PAPSS1
Ampicillin
Plate 1 / C / 5 pWZL Neo Myr Flag CSNK1G2
Ampicillin
Plate 1 / C / 6 pWZL Neo Myr Flag PCTK1
Ampicillin
Plate 1 / C / 7 pWZL Neo Myr Flag DAK
Ampicillin
Plate 1 / C / 8 pWZL Neo Myr Flag BMX
Ampicillin
Plate 1 / C / 9 pWZL Neo Myr Flag TAOK3
Ampicillin
Plate 1 / C / 10 pWZL Neo Myr Flag PRKCZ
Ampicillin
Plate 1 / C / 11 pWZL Neo Myr Flag CALM2
Ampicillin
Plate 1 / C / 12 pWZL Neo Myr Flag CDC2
Ampicillin
Plate 1 / D / 1 pWZL Neo Myr Flag RIOK3
Ampicillin
Plate 1 / D / 2 pWZL Neo Myr Flag GALK2
Ampicillin
Plate 1 / D / 3 pWZL Neo Myr Flag PIP5K1A
Ampicillin
Plate 1 / D / 4 pWZL Neo Myr Flag NEK6
Ampicillin
Plate 1 / D / 5 pWZL Neo Myr Flag DGKG
Ampicillin
Plate 1 / D / 6 pWZL Neo Myr Flag CDK2
Ampicillin
Plate 1 / D / 7 pWZL Neo Myr Flag CDK7
Ampicillin
Plate 1 / D / 8 pWZL Neo Myr Flag CKMT1A
Ampicillin
Plate 1 / D / 9 pWZL Neo Myr Flag GCK
Ampicillin
Plate 1 / D / 10 pWZL Neo Myr Flag CLK2
Ampicillin
Plate 1 / D / 11 pWZL Neo Myr Flag RPS6KA5
Ampicillin
Plate 1 / D / 12 pWZL Neo Myr Flag RPS6KA2
Ampicillin
Plate 1 / E / 1 pWZL Neo Myr Flag RET
Ampicillin
Plate 1 / E / 2 pWZL Neo Myr Flag FASTK
Ampicillin
Plate 1 / E / 3 pWZL Neo Myr Flag CDK4
Ampicillin
Plate 1 / E / 4 pWZL Neo Myr Flag MAPK14
Ampicillin
Plate 1 / E / 5 pWZL Neo Myr Flag LIMK1
Ampicillin
Plate 1 / E / 6 pWZL Neo Myr Flag BLK
Ampicillin
Plate 1 / E / 7 pWZL Neo Myr Flag MAP3K7
Ampicillin
Plate 1 / E / 8 pWZL Neo Myr Flag PKM2
Ampicillin
Plate 1 / E / 9 pWZL Neo Myr Flag TYK2
Ampicillin
Plate 1 / E / 10 pWZL Neo Myr Flag MKNK1
Ampicillin
Plate 1 / E / 11 pWZL Neo Myr Flag PRKRA
Ampicillin
Plate 1 / E / 12 pWZL Neo Myr Flag MAPK13
Ampicillin
Plate 1 / F / 1 pWZL Neo Myr Flag AURKA
Ampicillin
Plate 1 / F / 2 pWZL Neo Myr Flag PLAU
Ampicillin
Plate 1 / F / 3 pWZL Neo Myr Flag PCTK3
Ampicillin
Plate 1 / F / 4 pWZL Neo Myr Flag OXSR1
Ampicillin
Plate 1 / F / 5 pWZL Neo Myr Flag MAP3K6
Ampicillin
Plate 1 / F / 6 pWZL Neo Myr Flag RPS6KB2
Ampicillin
Plate 1 / F / 7 pWZL Neo Myr Flag VRK2
Ampicillin
Plate 1 / F / 8 pWZL Neo Myr Flag FGR
Ampicillin
Plate 1 / F / 9 pWZL Neo Myr Flag RIOK2
Ampicillin
Plate 1 / F / 10 pWZL Neo Myr Flag PCK2
Ampicillin
Plate 1 / F / 11 pWZL Neo Myr Flag LIMK2
Ampicillin
Plate 1 / F / 12 pWZL Neo Myr Flag ACVR1
Ampicillin
Plate 1 / G / 1 pWZL Neo Myr Flag STK3
Ampicillin
Plate 1 / G / 2 pWZL Neo Myr Flag AMHR2
Ampicillin
Plate 1 / G / 3 pWZL Neo Myr Flag GK2
Ampicillin
Plate 1 / G / 4 pWZL Neo Myr Flag ADCK5
Ampicillin
Plate 1 / G / 5 pWZL Neo Myr Flag CSNK1A1L
Ampicillin
Plate 1 / G / 6 pWZL Neo Myr Flag MAP2K7
Ampicillin
Plate 1 / G / 7 pWZL Neo Myr Flag PIP5K3
Ampicillin
Plate 1 / G / 8 pWZL Neo Myr Flag NEK11
Ampicillin
Plate 1 / G / 9 pWZL Neo Myr Flag NEK3
Ampicillin
Plate 1 / G / 10 pWZL Neo Myr Flag GRK6
Ampicillin
Plate 1 / G / 11 pWZL Neo Myr Flag HIPK1
Ampicillin
Plate 1 / G / 12 pWZL Neo Myr Flag PMVK
Ampicillin
Plate 1 / H / 1 pWZL Neo Myr Flag PACSIN1
Ampicillin
Plate 1 / H / 2 pWZL Neo Myr Flag ILK
Ampicillin
Plate 1 / H / 3 pWZL Neo Myr Flag CLK1
Ampicillin
Plate 1 / H / 4 pWZL Neo Myr Flag STK33
Ampicillin
Plate 1 / H / 5 pWZL Neo Myr Flag SYK
Ampicillin
Plate 1 / H / 6 pWZL Neo Myr Flag ADRBK1
Ampicillin
Plate 1 / H / 7 pWZL Neo Myr Flag RPS6KL1
Ampicillin
Plate 1 / H / 8 pWZL Neo Myr Flag HK2
Ampicillin
Plate 1 / H / 9 pWZL Neo Myr Flag CERK
Ampicillin
Plate 1 / H / 10 pWZL Neo Myr Flag PDXK
Ampicillin
Plate 1 / H / 11 pWZL Neo Myr Flag ADRBK2
Ampicillin
Plate 1 / H / 12 pWZL Neo Myr Flag PIK3R3
Ampicillin
Plate 2 / A / 1 pWZL Neo Myr Flag PCTK2
Ampicillin
Plate 2 / A / 2 pWZL Neo Myr Flag MAPKAP1
Ampicillin
Plate 2 / A / 3 pWZL Neo Myr Flag ULK4
Ampicillin
Plate 2 / A / 4 pWZL Neo Myr Flag ITK
Ampicillin
Plate 2 / A / 5 pWZL Neo Myr Flag BTK
Ampicillin
Plate 2 / A / 6 pWZL Neo Myr Flag RPSK6A3
Ampicillin
Plate 2 / A / 7 pWZL Neo Myr Flag EPHA4
Ampicillin
Plate 2 / A / 8 pWZL Neo Myr Flag MAP3K8
Ampicillin
Plate 2 / A / 9 pWZL Neo Myr Flag AKT3
Ampicillin
Plate 2 / A / 10 pWZL Neo Myr Flag CAMKK1
Ampicillin
Plate 2 / A / 11 pWZL Neo Myr Flag GK
Ampicillin
Plate 2 / A / 12 pWZL Neo Myr Flag YES1
Ampicillin
Plate 2 / B / 2 pWZL Neo Myr Flag DLG5
Ampicillin
Plate 2 / B / 3 pWZL Neo Myr Flag RPS6KA6
Ampicillin
Plate 2 / B / 4 pWZL Neo Myr Flag CAMK4
Ampicillin
Plate 2 / B / 5 pWZL Neo Myr Flag HCK
Ampicillin
Plate 2 / B / 6 pWZL Neo Myr Flag RPS6KB1
Ampicillin
Plate 2 / B / 7 pWZL Neo Myr Flag MAPK6
Ampicillin
Plate 2 / B / 8 pWZL Neo Myr Flag TEC
Ampicillin
Plate 2 / B / 9 pWZL Neo Myr Flag PRKCD
Ampicillin
Plate 2 / B / 10 pWZL Neo Myr Flag PIK4CA
Ampicillin
Plate 2 / B / 11 pWZL Neo Myr Flag PIK3CB
Ampicillin
Plate 2 / B / 12 pWZL Neo Myr Flag VRK3
Ampicillin
Plate 2 / C / 1 pWZL Neo Myr Flag PI4K2B
Ampicillin
Plate 2 / C / 2 pWZL Neo Myr Flag FRK
Ampicillin
Plate 2 / C / 3 pWZL Neo Myr Flag PRKAA1
Ampicillin
Plate 2 / C / 4 pWZL Neo Myr Flag GRK5
Ampicillin
Plate 2 / C / 5 pWZL Neo Myr Flag PLK2
Ampicillin
Plate 2 / C / 6 pWZL Neo Myr Flag PFKM
Ampicillin
Plate 2 / C / 7 pWZL Neo Myr Flag MOBKL2A
Ampicillin
Plate 2 / C / 8 pWZL Neo Myr Flag AAK1
Ampicillin
Plate 2 / C / 9 pWZL Neo Myr Flag TSSK1B
Ampicillin
Plate 2 / C / 10 pWZL Neo Myr Flag MAST1
Ampicillin
Plate 2 / C / 11 pWZL Neo Myr Flag PDIK1L
Ampicillin
Plate 2 / C / 12 pWZL Neo Myr Flag NADK
Ampicillin
Plate 2 / D / 1 pWZL Neo Myr Flag DYRK4
Ampicillin
Plate 2 / D / 2 pWZL Neo Myr Flag MVK
Ampicillin
Plate 2 / D / 3 pWZL Neo Myr Flag PAK4
Ampicillin
Plate 2 / D / 4 pWZL Neo Myr Flag TESK1
Ampicillin
Plate 2 / D / 5 pWZL Neo Myr Flag MAPK12
Ampicillin
Plate 2 / D / 6 pWZL Neo Myr Flag ITPKB
Ampicillin
Plate 2 / D / 7 pWZL Neo Myr Flag IHPK2
Ampicillin
Plate 2 / D / 8 pWZL Neo Myr Flag SPHK2
Ampicillin
Plate 2 / D / 9 pWZL Neo Myr Flag PIK3CG
Ampicillin
Plate 2 / D / 10 pWZL Neo Myr Flag STK32C
Ampicillin
Plate 2 / D / 11 pWZL Neo Myr Flag CKB
Ampicillin
Plate 2 / D / 12 pWZL Neo Myr Flag ITPK1
Ampicillin
Plate 2 / E / 1 pWZL Neo Myr Flag GAK
Ampicillin
Plate 2 / E / 2 pWZL Neo Myr Flag PRKACG
Ampicillin
Plate 2 / E / 3 pWZL Neo Myr Flag STK40
Ampicillin
Plate 2 / E / 4 pWZL Neo Myr Flag PDPK1
Ampicillin
Plate 2 / E / 5 pWZL Neo Myr Flag HK1
Ampicillin
Plate 2 / E / 6 pWZL Neo Myr Flag PIK3R5
Ampicillin
Plate 2 / E / 7 pWZL Neo Myr Flag PNKP
Ampicillin
Plate 2 / E / 8 pWZL Neo Myr Flag PRKAG2
Ampicillin
Plate 2 / E / 9 pWZL Neo Myr Flag NUAK2
Ampicillin
Plate 2 / E / 10 pWZL Neo Myr Flag SNF1LK
Ampicillin
Plate 2 / E / 11 pWZL Neo Myr Flag HK3
Ampicillin
Plate 2 / E / 12 pWZL Neo Myr Flag PIP5K2A
Ampicillin
Plate 2 / F / 1 pWZL Neo Myr Flag PIP5K1B
Ampicillin
Plate 2 / F / 2 pWZL Neo Myr Flag PBK
Ampicillin
Plate 2 / F / 3 pWZL Neo Myr Flag STK32B
Ampicillin
Plate 2 / F / 4 pWZL Neo Myr Flag CMPK
Ampicillin
Plate 2 / F / 5 pWZL Neo Myr Flag ADPGK
Ampicillin
Plate 2 / F / 6 pWZL Neo Myr Flag MAP3K14
Ampicillin
Plate 2 / F / 7 pWZL Neo Myr Flag CSNK1G1
Ampicillin
Plate 2 / F / 8 pWZL Neo Myr Flag TNK2
Ampicillin
Plate 2 / F / 9 pWZL Neo Myr Flag NTRK3
Ampicillin
Plate 2 / F / 10 pWZL Neo Myr Flag DGUOK
Ampicillin
Plate 2 / F / 11 pWZL Neo Myr Flag MOBKL1A
Ampicillin
Plate 2 / F / 12 pWZL Neo Myr Flag NME7
Ampicillin
Plate 2 / G / 1 pWZL Neo Myr Flag FGFR1
Ampicillin
Plate 2 / G / 2 pWZL Neo Myr Flag PFKL
Ampicillin
Plate 2 / G / 3 pWZL Neo Myr Flag PKN1
Ampicillin
Plate 2 / G / 4 pWZL Neo Myr Flag PLK4
Ampicillin
Plate 2 / G / 5 pWZL Neo Myr Flag CKMT2
Ampicillin
Plate 2 / G / 6 pWZL Neo Myr Flag RIOK1
Ampicillin
Plate 2 / G / 7 pWZL Neo Myr Flag STK32B
Ampicillin
Plate 2 / G / 8 pWZL Neo Myr Flag ADCK4
Ampicillin
Plate 2 / G / 9 pWZL Neo Myr Flag STK4
Ampicillin
Plate 2 / G / 10 pWZL Neo Myr Flag TIE1
Ampicillin
Plate 2 / G / 11 pWZL Neo Myr Flag PTK2
Ampicillin
Plate 2 / G / 12 pWZL Neo Myr Flag SRPK2
Ampicillin
Plate 2 / H / 1 pWZL Neo Myr Flag PRKACB
Ampicillin
Plate 2 / H / 2 pWZL Neo Myr Flag MAP2K6
Ampicillin
Plate 2 / H / 3 pWZL Neo Myr Flag PRKAR2A
Ampicillin
Plate 2 / H / 4 pWZL Neo Myr Flag AXL
Ampicillin
Plate 2 / H / 5 pWZL Neo Myr Flag PDK1
Ampicillin
Plate 2 / H / 6 pWZL Neo Myr Flag CAMK1G
Ampicillin
Plate 2 / H / 7 pWZL Neo Myr Flag TBK1
Ampicillin
Plate 2 / H / 8 pWZL Neo Myr Flag STK32A
Ampicillin
Plate 2 / H / 9 pWZL Neo Myr Flag TSSK6
Ampicillin
Plate 2 / H / 10 pWZL Neo Myr Flag CSNK1E
Ampicillin
Plate 2 / H / 11 pWZL Neo Myr Flag CAMK2D
Ampicillin
Plate 2 / H / 12 pWZL Neo Myr Flag CAMK2B
Ampicillin
Data calculated @ 2016-12-05

Kit Plate Map - #1000000012

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