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Plasmid-Reference_book.png Genetic Code, Codon Table, and Epitope Tags


Amino Acids

Alanine Ala A GCU, GCC, GCA, GCG
Arginine Arg R CGU, CGC, CGA, CGG, AGA, AGG
Asparagine Asn N AAU, AAC
Aspartic acid Asp D GAU, GAC
Cysteine Cys C UGU, UGC
Glutamine Gln Q CAA, CAG
Glutamic Acid Glu E GAA, GAG
Glycine Gly G GGU, GGC, GGA, GGG
Histidine His H CAU, CAC
Isoleucine Ile I AUU, AUC, AUA
Leucine Leu L UUA, UUG, CUU, CUC, CUA, CUG
Lysine Lys K AAA, AAG
Methionine Met M AUG
Phenylalanine Phe F UUU, UUC
Proline Pro P CCU, CCC, CCA, CCG
Serine Ser S UCU, UCC, UCA, UCG, AGU,AGC
Threonine Thr T ACU, ACC, ACA, ACG
Tryptophan Trp W UGG
Tyrosine Tyr Y UAU, UAC
Valine Val V GUU, GUC, GUA, GUG
Start AUG*
Stop UAG (amber), UGA (opal), UAA (ochre)

*AUG is the most common start codon. Alternative start codons include CUG in eukaryotes and GUG in prokaryotes.


RNA Codon Table

Second
Position
U C A G
U UUU (Phe)
UUC (Phe)
UUA (Leu)
UUG (Leu)
UCU (Ser)
UCC (Ser)
UCA (Ser)
UCG (Ser)
UAU (Tyr)
UAC (Tyr)
UAA (Stop)
UAG (Stop)
UGU (Cys)
UGC (Cys)
UGA (Stop)
UGG (Trp)
U
C
A
G
First
Position
(5' end)
C CUU (Leu)
CUC (Leu)
CUA (Leu)
CUG (Leu)
CCU (Pro)
CCC (Pro)
CCA (Pro)
CCG (Pro)
CAU (His)
CAC (His)
CAA (Gln)
CAG (Gln)
CGU (Arg)
CGC (Arg)
CGA (Arg)
CGG (Arg)
U
C
A
G
Third
Position
(3' end)
A AUU (Ile)
AUC (Ile)
AUA (Ile)
AUG (Met/Start)
ACU (Thr)
ACC (Thr)
ACA (Thr)
ACG (Thr)
AAU (Asn)
AAC (Asn)
AAA (Lys)
AAG (Lys)
AGU (Ser)
AGC (Ser)
AGA (Arg)
AGG (Arg)
U
C
A
G
G GUU (Val)
GUC (Val)
GUA (Val)
GUG (Val)
GCU (Ala)
GCC (Ala)
GCA (Ala)
GCG (Ala)
GAU (Asp)
GAC (Asp)
GAA (Glu)
GAG (Glu)
GGU (Gly)
GGC (Gly)
GGA (Gly)
GGG (Gly)
U
C
A
G

Nucleotide Single Letter Codes

A Adenine
C Cytosine
G Guanine
T Thymine
U Uracil
B C, G, or T
D A, G, or T
H A, C, or T
K G or T
M A or C
N A, T, C, or G
R A or G
S C or G
V A, C, or G
W A or T
Y C or T

Common Epitope Tags

Tag Protein Sequence DNA Sequence*
FLAG DYKDDDDK GAC TAC AAA GAC GAT GAC GAC AAG
HA YPYDVPDYA TAC CCA TAC GAT GTT CCA GAT TAC GCT
His HHHHHH CAC CAC CAC CAC CAC CAC
Myc EQKLISEEDL GAA CAA AAA CTC ATC TCA GAA GAG GAT CTG
V5 GKPIPNPLLGLDST
Xpress DLDDDDK or DLYDDDDK
Thrombin LVPRGS
BAD (Biotin Acceptor Domain) GLNDIFEAQKIEWHE
Factor Xa IEGR or IDGR
VSVG YTDIEMNRLGK
SV40 NLS PKKKRKV or PKKKRKVG
Protein C EDQVDPRLIDGK
S Tag KETAAAKFERQHMDS
OneStrap SAWSHPQFEK2GGSAWSHPQFEK
SB1 PRPSNKRLQQ

*Due to the degenerate nature of the genetic code, the given sequence is one of a number of sequences that can encode the same epitope tag.