We recently published an article in Nature Biotechnology highlighting Addgene's plasmid sharing model. The article focused on Addgene's growth in recent years and how the collection is distributed to labs around the world. In the correspondence, we had the opportunity to highlight some our collections most requested plasmids (and the labs that contributed them). Below, we acknowledge an extended list of Addgene depositors whose plasmids have been highly requested over the years.
We thank all our depositors for their contributions to the collection and their continued support!
| Depositor |
Institution |
Total Plasmid Distributions |
Most Requested Individual Plasmids |
| Bob Weinberg |
Whitehead Institute, Cambridge, USA |
9255 |
pCMV-VSV-G, pBABE-puro, pCMV-dR8.2 dvpr |
| Didier Trono |
EPFL, Lausanne, Switzerland |
8854 |
pMD2.G, psPAX2, pLVTHM |
| Shinya Yamanaka |
Kyoto University, Kyoto, Japan |
8102 |
pMXs-hc-MYC, pMXs-hKLF4 , pMXs-hSOX2 |
| Bill Hahn |
Harvard Medical School, Boston, USA |
5294 |
pBABE-GFP,pBABE puro H-Ras V12 |
| Connie Cepko |
Harvard Medical School, Boston, USA |
5063 |
pCAG-ERT2CreERT2, pCAG-Cre:GFP, pCAG-CreERT2, pCAG-Cre |
| David Sabatini |
MIT, Cambridge, USA |
4582 |
scramble shRNA, myc-mTOR, myc-Rictor corrected, myc-Raptor |
| Bert Vogelstein |
Johns Hopkins, Baltimore, USA |
4311 |
WWP-Luc (p21/WAF1 promoter), PG13-luc (wt p53 binding sites), SBE4-Luc, pCMV-Neo-Bam p53 wt |
| Eric Campeau |
UMass, Worcester, USA |
3780 |
pLenti CMV Puro DEST (w118-1), pLenti CMV TetR Blast (716-1), pLenti CMV GFP Puro (658-5), pLenti CMV/TO Puro DEST (670-1) |
| William Sellers |
DFCI, Boston, USA |
3515 |
pcDNA3 Myr HA Akt1, pCMVb p300 HA |
| James Thomson |
University of Wisconsin, Madison, USA |
3334 |
pSin-EF2-LIN28-Pur, pSin-EF2-Nanog-Pur, pSin-EF2-Oct4-Pur, pSin-EF2-Sox2-Pur |
| Rudolf Jaenisch |
Whitehead Institute, Cambridge, USA |
2880 |
FUW-M2rtTA, TetO-FUW-OSKM |
| Tyler Jacks |
MIT, Cambridge, USA |
2772 |
pSico, Lox-Stop-Lox TOPO, GFP-p53 |
| Keith Joung |
MIT, Cambridge, USA |
2535 |
pMLM290, pMLM292, pBAC-lacZ, strain KJBAC1, Zinc Finger Kits, TALEN Kits |
| Wade Harper |
Harvard Medical School, Boston, USA |
2430 |
pcDNA3-DN-hCUL1-FLAG, also available (hCUL2), (hCUL3), (hCUL4A), (hCUL4B), and (hCUL5) |
| Patrick Aebischer |
EPFL, Lausanne, Switzerland |
2127 |
pLVCT-tTR-KRAB, pLVPT-tTR-KRAB |
| Bruce Spiegelman |
Harvard Medical School, Boston, USA |
2110 |
PPRE X3-TK-luc, PGC-1 alpha promoter 2kb luciferase |
| Doug Golenbock |
UMass, Worcester, USA |
1865 |
pcDNA3-EGFP, pcDNA3-YFP, pcDNA3-CFP, pcDNA3-mRFP |
| Randall Moon |
University of Washington, Seattle, USA |
1831 |
M50 Super 8x TOPFlash, M51 Super 8x FOPFlash (TOPFlash mutant) |
| David Root |
Broad Institute, Cambridge, USA |
1798 |
pLKO.1 - TRC cloning vector, pLKO.1 - TRC control |
| Ted Dawson |
Johns Hopkins, Baltimore, USA |
1790 |
pRK5-HA-Ubiquitin-WT, pRK5-HA-Ubiquitin-KO |
| Anjana Rao |
La Jolla Institute for Allergy & Immunology, La Jolla, USA |
1788 |
IKK-2 S177E S181E, NFAT/AP-1 3x luciferase |
| Michael Greenberg |
Harvard Medical School, Cambridge, USA |
1773 |
Flag-SIRT1, FHRE-Luc, Flag-SIRT1 H363Y, FLAG-FOXO3a WT |
| Eric Verdin |
University of California, San Francisco, USA |
1686 |
HDAC1 Flag, HDAC6 Flag, SIRT2 Flag, HDAC3 Flag, HDAC4 Flag |
| Gary Bokoch |
Scripps Research Institute, La Jolla, USA |
1653 |
pcDNA3-EGFP-RhoA-T19N, pcDNA3-EGFP-RhoA-Q63L, pcDNA3-EGFP-Rac1-Q61L, pcDNA3-EGFP-Rac1-T17N |
| William Kaelin |
Harvard Medical School, Cambridge, USA |
1653 |
HA-HIF1alpha-pcDNA3, HA-HIF1alpha P402A/P564A-pcDNA3, Luciferase-pcDNA3, HA-HIF2alpha-pcDNA3 |
| Thomas Tuschl |
Rockefeller University, New York, USA |
1629 |
pIRESneo-FLAG/HA Ago2 corrected, pIRESneo-FLAG/HA Ago1, pIRESneo-FLAG/HA Ago4, pIreSneo-FLAG/HA Ago3Y |
| Klaus Hahn |
UNC-Chapel Hill School of Medicine, Chapel Hill, USA |
1619 |
pcDNA3-EGFP-Cdc42(T17N), pTriEx-RhoA FLARE.sc Biosensor WT, pcDNA3-EGFP-Cdc42(wt) |
| Mien-Chie Hung |
University of Texas MD Anderson Cancer Center, Houston, USA |
2127 |
HER2 WT, pcDNA3 MDM2 WT, Flag p21 WT, Flag Snail WT,HA-Akt DN (K179M), HER2 CA (V659E) |
| Roger Davis |
UMass, Worcester, USA |
1550 |
pCDNA3 Flag Jnk1a1, pCDNA3-Flag MKK6(glu), pCDNA3 Flag Jnk1a1(apf), pCDNA3 Flag p38 alpha, pCDNA3 Flag MKK4, |
| Richard Pagano |
Mayo Clinic, Rochester, USA |
1547 |
DsRed-rab7 WT, DsRed-rab11 WT, GFP-rab7 WT, GFP-rab11 WT |
| Erik Jorgensen |
University of Utah, Salt Lake City, USA |
1529 |
pJL43.1 - Pglh-2::MosTase::glh-2utr, pCFJ90 - Pmyo-2::mCherry::unc-54utr, pCFJ104 - Pmyo-3::mCherry::unc-54, pGH8 - pRAB-3::mCherry::unc-54utr |
| Joan Massague |
Sloan-Kettering Cancer Center, New York, USA |
2127 |
pCMV5 human p27, pCMV5 Smad2-HA, pCMV5 BRGI-Flag, CS2 Flag-Smad2, pCMV5 Flag-Smad1 |
| Susan Lindquist |
MIT, Cambridge, USA |
1497 |
S. cerevisiae Advanced Gateway Destination Vectors, pBabe-c-myc-zeo, pAG426GPD-ccdB, pBabe-PDGF/B-zeo |
| Jeff Wrana |
Howard Hughes Medical Institute, Chevy Chase, USA |
1493 |
p3TP-lux, pCMV5-Smad7-HA, pCMV5B-TGFbeta receptor II wt, pCMV5B-Flag-Smad3, pCMV5B-Flag-Smurf1 wt |
| David Waugh |
National Cancer Institute, Frederick, USA |
1431 |
pRK793, pDEST-HisMBP, pRK1043, pDEST-periHisMBP, pRK603 |
| Robert Campbell |
University of Alberta, Edmonton, Canada |
1420 |
CMV-R-GECO1, pEBFP2-Nuc, pBad-EBFP2, CMV-GEM-GECO1, pmAmetrine-DEVD-tdTomato |
| Jean Zhao |
Dana Farber Cancer Institute, Boston, USA |
1360 |
pBabe puro HA PIK3CA H1047R, pBabe puro Myr HA PIK3CA, pBabe puro HA PIK3CA, pBabe puro HA PIK3CA E545K, pWZL-Neo-Myr-Flag-DEST |
| Peter Howley |
Dana Farber Cancer Institute, Boston, USA |
1301 |
p4489 Flag-betaTrCP, p1321 HPV-16 E6/E7, p3113 GST-p53, p1322 HPV-16 E6, p3868 HA-E6-AP |
| Yue Xiong |
University of North Carolina - Chapel Hill, Chapel Hill, USA |
1259 |
pCDNA3-Myc3-Nrf2, pcDNA3-myc-Skp2, pcDNA3-EGFP-C4-Nrf2, pcDNA3-HA2-Keap1, pCMV-myc3-HDM2 |
| Karl Deisseroth |
Stanford University, Stanford, USA |
1215 |
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA, pcDNA3.1/hChR2(H134R)-mCherry, pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA |
| Inder Verma |
Salk Institute for Biological Studies, La Jolla, USA |
1205 |
pCL-Eco, LV-Cre, LV-Lac, pCL-MFG-BRCA1 |
| Nico Dantuma |
Karolinska Institutet, Stockholm, Sweden |
1174 |
GFP-Ub, Ub-G76V-GFP, GFP-Ub KO, mRFP-Ub, GFP-Ub KO.G76V, Ub-R-GFP |
| Philippe Soriano |
Mount Sinai School of Medicine, New York, USA |
1143 |
pPGKFLPobpA, pPGKPhiC31obpA, PGKneoF2L2DTA , pFLPo, PGKdtabpA |
| Toren Finkel |
National Heart, Lung, and Blood Institute, Bethesda, USA |
1132 |
pcDNA4 myc PGC-1 alpha, NFAT luciferase reporter, pCruzHA SIRT1, pEGFP-LC3 (human), pTA-Luc SIRT1 promoter (-202), pEGFP-C1 NFAT3 |
| Edward Yeh |
University of Texas MD Anderson Cancer Center, Houston, USA |
1053 |
HA-Ubiquitin , pcDNA3/HA-SUMO3 (Sentrin 2), SRa-HA-SUMO2 (Sentrin 3), Flag-SENP1, HA-NEDD8 |
| John Blenis |
Harvard Medical School, Boston, USA |
1043 |
pcDNA3-HA-ERK2 WT, pRK7-HA-S6K1-WT, pcDNA3-myc-FLNa WT, pRK7-FLAG-TSC1 |
| Linzhao Cheng |
Johns Hopkins, Baltimore, USA |
1025 |
EF.hICN1.CMV.GFP, EF.CMV.RFP, EF.hICN1.Ubc.GFP, EF.STAT3C.Ubc.GFP, Ef1a_Large T-antigen_Ires_Puro |
