Addgene - pAC-Kan-alphaGal4 Plasmid Data

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Plasmid 13421: pAC-Kan-alphaGal4

Gene/insert name:		RNAP alpha-Gal4
Insert size (bp):		900
Species of gene(s):		Other
Vector backbone:		pACLU-Kan (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		3600
5' Sequencing primer:		AGA GCC TGA TAA AAA CGG TTA GCG (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Needs strain with lacIq
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		XL1 Blue
Principal Investigator:		Keith Joung
Terms and Licenses:		MTA

Comments: Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAT/CamR(144-0)

4 - 4367

ORF frame 1

1410 - 595

KanR2

1410 - 595

p15A_origin

1738 - 2650

lac_promoter

2999 - 3022

M13_pUC_rev_primer

3042 - 3064

M13_reverse_primer

3063 - 3081

ORF frame 3

3123 - 3998

Unique restriction sites

NcoI		1
NruI		1321
NheI		1740
SacII		1993
AatII		2691
MscI		3229
PstI		3458
PmlI		3506
EcoRI		3595
SalI		3639
NotI		3868
HpaI		3982
BclI		4104

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13421" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.