Addgene - pGP-FF Plasmid Data

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Plasmid 13480: pGP-FF

Gene/insert name:		none
Insert size (bp):		Unknown
Fusion proteins or tags:		Gal11P
Terminal:		N terminal on backbone
Vector backbone:		pGP-FF (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		3483
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		BsgI
Site destroyed during cloning:		No
5' Sequencing primer:		GGG GGA TTT CTG TTC ATG GGG G (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Needs strain with lacIq
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		XL1 Blue
Principal Investigator:		Keith Joung
Terms and Licenses:		MTA

Comments: Bacterial cell-based two-hybrid (B2H) expression vector. Encodes zinc finger-Gal11P hybrid protein. This plasmid is a corrected version of pGP-FB.

Note that the BsgI site (not pictured in the map below) cuts at base 420.

pGP-FF requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

lac_promoter

12 - 35

M13_pUC_rev_primer

55 - 77

f1_origin

581 - 887

AmpR_promoter

1080 - 1108

ORF frame 1

1150 - 2010

Ampicillin

1150 - 2010

pBR322_origin

2165 - 2784

pGEX_3_primer

3181 - 3203

ROP

3388 - 3197

Unique restriction sites

EcoRI		1
SalI		44
HindIII		351
NotI		360
EagI		360
XbaI		392
BamHI		427
AseI		1762
NdeI		3004
PvuII		3236

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13480" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.