Addgene - L4020 Plasmid Data

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Fire Lab C. elegans Vector Kit

Andrew Fire Lab Plasmids

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Plasmid 1616: L4020

Insert size (bp):		Unknown
Species of gene(s):		Other
Fusion proteins or tags:		rf8134, IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSM, IVSL, decoy
Terminal:
Type of vector:		Worm expression
Backbone size (bp):		4718
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Andrew Fire
Terms and Licenses:		MTA

Comments: See Fire Lab Vector Kit Documentation 1999. Fire Lab Miniprep Number pPD121.89, Ligation number L4020.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

rf81.34

19 - 39

SynIVS.A

60 - 101

SV40.NLS

135 - 161

SV40.NLS

177 - 203

SV40.NLS

219 - 245

SV40.NLS

261 - 287

SynIVS.R

474 - 524

gfp.S65C

546 - 565

ORF frame 3

9 - 1172

SynIVS.S

679 - 729

GFP(w/ gaps)

303 - 1163

SynIVS.T

894 - 944

SynIVS.M

1186 - 1236

SynIVS.L

1313 - 1363

Amp_promoter

2491 - 2519

ORF frame 2

2561 - 3421

Ampicillin

2561 - 3421

pBR322_origin

3576 - 4195

lacI.C

4411 - 4437

lac_promoter

4504 - 4533

M13r_primer

4568 - 4586

decoy

4600 - 4650

Unique restriction sites

HindIII		1
PstI		17
BamHI		29
SmaI		36
SacI		126
AgeI		290
NcoI		468
XhoI		566
EcoRI		1236
ApaI		2114
FspI		3125

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 1616" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.