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LCAG-ChR2-GFP
(Plasmid #22049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22049 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    LB2
  • Backbone manufacturer
    Patrick Stern
  • Backbone size w/o insert (bp) 8969
  • Vector type
    Mammalian Expression, Mouse Targeting, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ChR2-GFP
  • Alt name
    channelrhodopsin-2
  • Species
    C. reinhardtii
  • Insert Size (bp)
    1677
  • Mutation
    N/A
  • GenBank ID
    EU714030
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5' ATTGATTTGATACCGCGGGC 3'
  • 3′ sequencing primer 5' CAAAGAGATAGCTTGGTATT 3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PLEASE CONTACT ED BOYDEN ([email protected]) FOR DETAILS ON THIS REAGENT AND FURTHER NOVEL REAGENTS IN THIS LINE.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LCAG-ChR2-GFP was a gift from Edward Boyden (Addgene plasmid # 22049)
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