Addgene - pEGFP-NMHC II-C Plasmid Data

Gene/insert name:		NMHC II-C
Alternative names:		nonmuscle myosin II-C heavy chain
Insert size (bp):		6400
Gene/insert aliases:		Myh14, II-C, NHMCII, NMHC II-C, 2400004E04Rik
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		EGFP
Terminal:		C terminal on backbone
Vector backbone:		pEGFP-N3 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		4700
Cloning site 5':		SalI
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		EGFP-N (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Robert Adelstein
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. Golomb E et al. (J Biol Chem. 2004 Jan 23. 279(4):2800-8. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 10843" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.