Addgene - pTRE-GFP-NMHC II-A Plasmid Data

Gene/insert name:		NMHC II-A
Alternative names:		nonmuscle myosin II-A heavy chain
Insert size (bp):		6000
Gene/insert aliases:		MYH9, MHA, FTNS, EPSTS, DFNA17, NMMHCA, MGC104539, NMHC-II-A
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		EGFP
Terminal:		N terminal on backbone
Vector backbone:		pEGFP-C3 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		4700
Cloning site 5':		HindIII
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		Yes
5' Sequencing primer:		EGFP-C (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Robert Adelstein
Terms and Licenses:		MTA, Tet Systems LULL

Comments: The CMV promoter in pEGFP-C3 is replaced by a tet-responsive promoter amplified from pTRE (Clontech). NMHC II-A can be released by HindIII/SpeI digestion.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: Conditional expression of a truncated fragment of nonmuscle myosin II-A alters cell shape but not cytokinesis in HeLa cells. Wei Q et al. (Mol Biol Cell. 2000 Oct . 11(10):3617-27. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 10844" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.