Addgene - pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch Plasmid Data

Browse > Patrick Aebischer > Raoul et al > pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

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NCBI: SOD1

SOD1 antibodies

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pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1

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Plasmid 10881: pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch

Gene/insert name:		H1-shSOD1mismatch
Alternative names:		SOD1
Insert size (bp):		394
Gene/insert aliases:		SOD1, ALS, SOD, ALS1, IPOA, homodimer
Species of gene(s):		H. sapiens (human)
Vector backbone:		pCCL-cPPT-PGK-EGFP-WPRE (Search Vector Database)
Type of vector:		Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp):		7751
Cloning site 5':		ClaI
Site destroyed during cloning:		Yes
Cloning site 3':		ClaI
Site destroyed during cloning:		Yes
5' Sequencing primer:		H1 specific primer (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Sequence:		View sequence
Plasmid Provided In:		Stbl3
Principal Investigator:		Patrick Aebischer
Terms and Licenses:		MTA

Comments: shSOD1 mismatch sense oligonucleotide: 5'-GATCCCCAAG GATTAATAGA GGCATGTTCA AGAGACATGC CTCTATTAAT CCTTTTTTTG GAAA-3'.

Please visit LentiWeb (http://www.lentiweb.com) for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Lentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALS. Raoul C et al. (Nat Med. 2005 Apr . 11(4):423-8. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 10881" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.