Addgene - pHaMDRwt Plasmid Data

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M14758

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Plasmid 10957: pHaMDRwt

Gene/insert name:		MDR1
Insert size (bp):		4000
GenBank/Entrez ID of insert:		M14758
Species of gene(s):		H. sapiens (human)
Vector backbone:		pHa (Search Vector Database)
Type of vector:		Mammalian expression,Retroviral
Backbone size (bp):		11000
Cloning site 5':		SacII
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Michael Gottesman
Terms and Licenses:		MTA

Comments: Full-length cDNA, has the wild-type glycine at position 185 on P-glycoprotein. The "Ha" in pHaMDRwt stand for the Harvey sarcoma virus. Vector can confer the complete multidrug-resistance phenotype to various drug sensitive cell lines.

Figure legend for author's map: Structure of retroviral vector carrying MDR] cDNA. sites: B, BamHI; E, EcoRI; H, HindIII; S, Stu I; X, Xho I. bp, Base pairs; amp, ampicillin resistance gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: A retrovirus carrying an MDR1 cDNA confers multidrug resistance and polarized expression of P-glycoprotein in MDCK cells. Pastan I et al. (Proc Natl Acad Sci U S A. 1988 Jun . 85(12):4486-90. Pubmed)

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