Addgene - p079 pPNT6 Plasmid Data

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Ramesh Shivdasani Lab Plasmids

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Plasmid 11072: p079 pPNT6

Gene/insert name:		na
Insert size (bp):		Unknown
Vector backbone:		pPNT6 (Search Vector Database)
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		7500
5' Sequencing primer:		M13R (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Ramesh Shivdasani
Terms and Licenses:		MTA

Comments: Targeting vector with Neo flanked by 2 LoxP sequences. Also called pPNT3LoxP2. Ligation of Neo Cassette from pPNT3.LoxP5' cut AscI/XbaI into pPNT3.LoxP3' cut AscI/XbaI. The resulting plasmid has Neomycin Cassette flanked with 2 LoxP sequences oriented in the same direction.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

loxP

52 - 85

MSCV_rev_primer

166 - 143

NeoR/KanR

618 - 1418

ORF frame 3

618 - 1421

loxP

1916 - 1949

MSCV_rev_primer

2082 - 2059

ORF frame 3

2619 - 3749

TK_PA_terminator

3734 - 4004

M13_forward20_primer

4834 - 4818

M13_pUC_fwd_primer

4849 - 4827

lacZ_a

4827 - 4970

pGEX_3_primer

5162 - 5184

AmpR_promoter

5343 - 5371

ORF frame 1

5413 - 6273

Ampicillin

5413 - 6273

pBR322_origin

6428 - 7047

lac_promoter

7356 - 7385

M13_pUC_rev_primer

7399 - 7421

M13_reverse_primer

7420 - 7438

T3_promoter

7455 - 7474

Unique restriction sites

NotI		2
SalI		13
KpnI		36
ClaI		1427
XbaI		1905
BamHI		1955
HpaI		1966
XhoI		1984
NheI		1993
EcoRI		2014
NruI		2910
SmaI		3726
HindIII		4809
NdeI		5026

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11072" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.