Addgene - pCAG-GFP Plasmid Data

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Plasmid 11150: pCAG-GFP

Gene/insert name:		EGFP from Aequorea victoria
Alternative names:		GFP
		green fluorescent protein
Insert size (bp):		772
Vector backbone:		pCAGEN (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4779
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		NotI
Site destroyed during cloning:		No
5' Sequencing primer:		pCAG-F (List of Sequencing Primers)
3' Sequencing primer:		EGFP-N
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		EGFP was from pEGFP-N1 (Clontech).
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Connie Cepko
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

84 - 371

ORF frame 1

1533 - 802

pCAG_F_primer

1678 - 1697

EGFP_N_primer

1835 - 1814

EGFP

1769 - 2485

ORF frame 2

1769 - 2488

ORF frame 1

1642 - 2622

ORF frame 2

2626 - 1706

EGFP_C_primer

2422 - 2443

rb_glob_PA_terminator

2507 - 3008

M13_reverse_primer

3068 - 3050

M13_pUC_rev_primer

3089 - 3067

lac_promoter

3132 - 3103

SV40_origin

3273 - 3350

SV40_promoter

3238 - 3417

SV40pro_F_primer

3335 - 3354

SV40_PA_terminator

3431 - 3562

EBV_rev_primer

3519 - 3538

pBR322_origin

4405 - 3786

ORF frame 3

5420 - 4560

Ampicillin

5420 - 4560

AmpR_promoter

5490 - 5462

Unique restriction sites

SpeI		18
XbaI		1623
EcoRI		1719
KpnI		1739
SmaI		1748
AgeI		1756
NotI		2491
MscI		2543
BglII		2572
HindIII		3036
StuI		3406
FspI		4855

Article: Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T et al. (Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11150" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.