Addgene - pgex6p-1mciap1mut Plasmid Data

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Plasmid 11484: pgex6p-1mciap1mut

Gene/insert name:		mciap1mut
Alternative names:		mciap1
Insert size (bp):		1700
Gene/insert aliases:		Birc2, Api1, Api2, IAP1, IAP2, MIHB, MIHC, Birc3, HIAP1, HIAP2, MIAP1, MIAP2, RNF48, cIAP1, cIAP2, mcIAP1, AW146227
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		582 to alanine
Fusion proteins or tags:		GST
Terminal:		N terminal on backbone
Vector backbone:		pGEX6p-1 (Search Vector Database)
Backbone manufacturer:		amersham
Type of vector:		Bacterial expression
Backbone size (bp):		4900
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		GGGCTGGCAAGCCACGTTTGGTGG (List of Sequencing Primers)
3' Sequencing primer:		TCCGGGAGCTGCATGTGTCAGAGG
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Jon Ashwell
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Posttranscriptional downregulation of c-IAP2 by the ubiquitin protein ligase c-IAP1 in vivo. Conze DB et al. (Mol Cell Biol. 2005 Apr . 25(8):3348-56. Pubmed)

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