Addgene - pDest-565 Plasmid Data

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Plasmid 11520: pDest-565

Gene/insert name:		N/A
Insert size (bp):		Unknown
Fusion proteins or tags:		His6-GST
Terminal:		N terminal on backbone
Vector backbone:		pDest-560 (Search Vector Database)
Backbone manufacturer:		PEL
Type of vector:		Bacterial expression,Gateway Destination Vector
Backbone size (bp):		8932
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin, Chloramphenicol
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Requires growth in a strain resistant to ccdB (DB3.1, ccdB Survival, or F')
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		GST gene from Promega pGEX vector
Sequence:		View sequence
Plasmid Provided In:		DB3.1
Principal Investigator:		Dominic Esposito
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

T7_promoter

2 - 20

lacO

20 - 47

T7_transl_en_RBS

65 - 81

GST

129 - 776

ORF frame 3

90 - 836

pGEX_5_primer

728 - 750

attR1

801 - 925

attR2

925 - 825

lac_promoter

950 - 973

ORF frame 2

1034 - 1693

CAT/CamR

1034 - 1693

pBR322_origin

1730 - 2349

ccdB

2967 - 3272

attR2

3313 - 3413

attR1

3420 - 3313

6xHis

3536 - 3553

rrnB_terminator

3599 - 3756

rrnB_T1_terminator

3722 - 3765

rrnB_T2_terminator

3897 - 3924

T7_Terminal_primer

3996 - 3978

T7_terminator

3997 - 4032

f1_origin

4093 - 4399

Ampicillin

5506 - 4646

AmpR_promoter

5577 - 5549

pBR322_origin

5664 - 6283

pGEX_3_primer

6682 - 6704

ROP

6889 - 6698

lacI

8552 - 7461

tet(636-888)

8644 - 8895

pBRrevBam_primer

8827 - 8846

Unique restriction sites

XbaI		48
NdeI		88
BstBI		514
EcoRI		1247
NcoI		1548
SmaI		3050
PstI		3305
SalI		3307
NheI		3444
HindIII		3461
PmlI		3488
XhoI		3530
FspI		4941
HpaI		7686
ApaI		7985
ClaI		8913

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11520" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.