Addgene - RCAS-myrAkt Plasmid Data

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Sandra Orsulic Lab Plasmids

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NC_000078

NCBI: Akt1

Akt1 antibodies

This is commonly
requested with

RCAS-K-ras

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Plasmid 11547: RCAS-myrAkt

Gene/insert name:		Protein Kinase B
Alternative names:		Akt1
		PKB
		PKBalpha
Insert size (bp):		1500
GenBank/Entrez ID of insert:		NC_000078
Gene/insert aliases:		Akt1, Akt, PKB, Rac, PKB/Akt, PKBalpha
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		myr signal
Terminal:		N terminal on insert
Fusion proteins or tags:		HA tag
Terminal:		N terminal on insert
Vector backbone:		RCAS (Search Vector Database)
Type of vector:		Retroviral
Backbone size (bp):		11600
Cloning site 5':		Pme1
Site destroyed during cloning:		Yes
Cloning site 3':		Pme1
Site destroyed during cloning:		Yes
5' Sequencing primer:		RCAS-F (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Sandra Orsulic
Terms and Licenses:		MTA

Comments: The RCAS vectors that carry oncogenes are harmless for mammalian cells, but can be oncogenic in avian cells since some avian cells express the receptor for the RCAS virus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Induction of ovarian cancer by defined multiple genetic changes in a mouse model system. Orsulic S et al. (Cancer Cell. 2002 Feb . 1(1):53-62. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11547" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.