Addgene - pcDNA3 proHB-EGF CS Plasmid Data

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NCBI: HBEGF

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Plasmid 11601: pcDNA3 proHB-EGF CS

Gene/insert name:		HB-EGF
Alternative names:		proHB-EGF
		sHB-EGF
Insert size (bp):		453
Gene/insert aliases:		HBEGF, DTR, DTS, DTSF, HEGFL
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		Constitutively secreted HB-EGF: C-terminal amino acid is Glu151.
Fusion proteins or tags:		myc
Terminal:		C terminal on backbone
Fusion proteins or tags:		His
Terminal:		C terminal on backbone
Vector backbone:		pcDNA3.1-myc/His(A) (Search Vector Database)
Backbone manufacturer:		Invitrogen
Type of vector:		Mammalian expression
Backbone size (bp):		5500
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		ApaI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Rosalyn Adam
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function. Adam RM et al. (Endocrinology. 2002 Dec . 143(12):4599-608. Pubmed)

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