Addgene - pIRES proHB-EGF NC Plasmid Data

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Plasmid 11604: pIRES proHB-EGF NC

Gene/insert name:		HB-EGF
Alternative names:		proHB-EGF
Insert size (bp):		667
Gene/insert aliases:		HBEGF, DTR, DTS, DTSF, HEGFL
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		Non-cleavable HB-EGF: Pro149 to Thr160 replaced with CD4 JM domain.
Vector backbone:		pIRESHyg2 (Search Vector Database)
Backbone manufacturer:		Clontech
Type of vector:		Mammalian expression
Backbone size (bp):		5800
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		NsiI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Hygromycin
Plasmid Provided In:		DH5a
Principal Investigator:		Rosalyn Adam
Terms and Licenses:		MTA

Comments: JM domain of HB-EGF replaced with JM domain of CD4 (PVENPLYTYDHT replaced with KVLPTWSTPVQP).

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function. Adam RM et al. (Endocrinology. 2002 Dec . 143(12):4599-608. Pubmed)

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