Addgene - pcDNA3 proHB-EGF NC Plasmid Data

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Plasmid 11605: pcDNA3 proHB-EGF NC

Gene/insert name:		HB-EGF
Alternative names:		proHB-EGF
Insert size (bp):		667
Gene/insert aliases:		HBEGF, DTR, DTS, DTSF, HEGFL
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		Non-cleavable HB-EGF: Pro149 to Thr160 replaced with CD4 JM domain.
Fusion proteins or tags:		myc
Terminal:		C terminal on backbone
Fusion proteins or tags:		His
Terminal:		C terminal on backbone
Vector backbone:		pcDNA3.1-myc/His(A) (Search Vector Database)
Backbone manufacturer:		Invitrogen
Type of vector:		Mammalian expression
Backbone size (bp):		5500
Cloning site 5':		HindIII
Site destroyed during cloning:		No
Cloning site 3':		ApaI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		We received a proHB-EGF plasmid incorporating the CD4 JM domain from Michael Klagsbrun, Vascular Biology Research program, Children's Hospital Boston. The original citation is Suzuki et al., 1997. J Biol Chem 272(50), 31730-737.
Plasmid Provided In:		DH5a
Principal Investigator:		Rosalyn Adam
Terms and Licenses:		MTA

Comments: JM domain of HB-EGF replaced with JM domain of CD4 (PVENPLYTYDHT replaced with KVLPTWSTPVQP). The original citation is Suzuki et al., 1997. J Biol Chem 272(50), 31730-737.

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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Article: Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress. Kim J et al. (Cancer Res. 2005 Sep 15. 65(18):8242-9. Pubmed)

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