Addgene - pGL3 NSE promoter Plasmid Data

Gene/insert name:		NSE promoter
Alternative names:		NSE
Insert size (bp):		1300
Gene/insert aliases:		ENO2, NSE
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		luciferase
Terminal:		C terminal on backbone
Vector backbone:		pGL3-Basic (Search Vector Database)
Backbone manufacturer:		Promega
Type of vector:		Mammalian expression,Luciferase
Backbone size (bp):		4800
Cloning site 5':		NheI
Site destroyed during cloning:		No
Cloning site 3':		BglII
Site destroyed during cloning:		No
5' Sequencing primer:		RVprimer3 (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Rosalyn Adam
Terms and Licenses:		MTA, Luciferase LULL

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Involvement of cholesterol-rich lipid rafts in interleukin-6-induced neuroendocrine differentiation of LNCaP prostate cancer cells. Kim J et al. (Endocrinology. 2004 Feb . 145(2):613-9. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11606" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.