Addgene - pGEX2T merlin 1-595 Plasmid Data

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NCBI: NF2

This is commonly
requested with

pGEX2T merlin 1-590

pGEX2T merlin 1-332

pGEX2T merlin 340-595

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Plasmid 11629: pGEX2T merlin 1-595

Gene/insert name:		merlin
Alternative names:		NF2
		isoform 1
Insert size (bp):		1785
Gene/insert aliases:		NF2, ACN, SCH, BANF
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		GST
Terminal:		N terminal on backbone
Vector backbone:		pGEX2T (Search Vector Database)
Backbone manufacturer:		Amersham
Type of vector:		Bacterial expression
Backbone size (bp):		4950
5' Sequencing primer:		pGEX5' (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Vijaya Ramesh
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Interdomain interaction of merlin isoforms and its influence on intermolecular binding to NHE-RF. Gonzalez-Agosti C et al. (J Biol Chem. 1999 Nov 26. 274(48):34438-42. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11629" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.