Addgene - pLVU-tTR-KRAB Plasmid Data

Gene/insert name:		hUbiquitin C, GFP, tTR-KRAB, no tetO
Insert size (bp):		Unknown
Vector backbone:		None (Search Vector Database)
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		11558
5' Sequencing primer:		See map (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		Stbl3
Principal Investigator:		Patrick Aebischer
Terms and Licenses:		MTA

Comments: pLVTHM and packaging plasmid for this system are also available at Addgene (http://www.addgene.org/rnaitools). Please visit Trono lab website (http://tronolab.epfl.ch) to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb (http://www.lentiweb.com) for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

CAG_enhancer

318 - 605

CMV_immearly_promoter

239 - 815

CMV_fwd_primer

772 - 792

HIV-1_5_LTR

835 - 1015

truncHIV-1_3_LTR

835 - 1015

HIV-1_psi_pack

1126 - 1170

RRE

1680 - 1913

ORF frame 1

1558 - 2445

cPPT

2444 - 2459

hUbC_promoter

2617 - 3833

EGFP_N_primer

3926 - 3905

EGFP

3860 - 4576

ORF frame 2

3860 - 4579

EGFP_C_primer

4513 - 4534

IRES

4606 - 5109

TetR

5187 - 5786

ORF frame 3

5172 - 6179

WPRE

6252 - 6839

pBluescriptKS_primer

6858 - 6842

ORF frame 1

6340 - 7389

cPPT

7026 - 7041

U3PPT

7026 - 7047

HIV-1_5_LTR

7363 - 7543

truncHIV-1_3_LTR

7363 - 7543

BGH_rev_primer

7586 - 7569

bGH_PA_terminator

7572 - 7799

f1_origin

7862 - 8168

pBABE_3_primer

8302 - 8282

SV40_enhancer

8503 - 8288

SV40_promoter

8300 - 8568

SV40_origin

8467 - 8544

SV40pro_F_primer

8529 - 8548

EM7_promoter

8662 - 8729

bleo

8730 - 9101

sh_ble

8730 - 9104

SV40_PA_terminator

9237 - 9356

EBV_rev_primer

9325 - 9344

M13_reverse_primer

9418 - 9400

M13_pUC_rev_primer

9439 - 9417

lac_promoter

9482 - 9453

pBR322_origin

10410 - 9791

ORF frame 2

11425 - 10565

Ampicillin

11425 - 10565

AmpR_promoter

11495 - 11467

Unique restriction sites

PstI		2414
BamHI		3835
AgeI		3847
XbaI		4592
BstBI		6224
XhoI		6854
MscI		8733
FspI		10860

Article: A versatile tool for conditional gene expression and knockdown. Szulc J et al. (Nat Methods. 2006 Feb . 3(2):109-16. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11645" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.