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Browse > Patrick Aebischer > Szulc et al > pLVUHshp53
Price: $65.00
 

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TP53 plasmids
Patrick Aebischer Lab Plasmids
Didier Trono Lab Plasmids
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NCBI: TP53
TP53 antibodies
 

This is commonly
requested with
pLVUHshp53- tTR-KRAB
pMD2.G
psPAX2
pLVUT-tTR-KRAB
pMKO.1 puro p53 shRNA 2
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Plasmid 11653: pLVUHshp53
Gene/insert name: GFP, shRNA against TP53
Alternative names: shRNA against p53
p53
Insert size (bp): Unknown
Gene/insert aliases: TP53, p53, LFS1, TRP53, FLJ92943
Species of gene(s): H. sapiens (human)
Vector backbone: None
(Search Vector Database)
Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp): 9931
5' Sequencing primer: See map  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Sequence:View sequence
Author's Map:View map
Plasmid Provided In:Stbl3
Principal Investigator:Patrick Aebischer
Terms and Licenses:MTA

Comments: Please visit Trono lab website to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb (http://www.lentiweb.com) for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
CAG_enhancer 318 - 605
CMV_immearly_promoter 239 - 815
CMV_fwd_primer 772 - 792
HIV-1_5_LTR 835 - 1015
truncHIV-1_3_LTR 835 - 1015
HIV-1_psi_pack 1126 - 1170
RRE 1680 - 1913
ORF frame 1 1558 - 2445
cPPT 2444 - 2459
hUbC_promoter 2617 - 3833
EGFP_N_primer 3926 - 3905
EGFP 3860 - 4576
ORF frame 2 3860 - 4579
EGFP_C_primer 4513 - 4534
WPRE 4622 - 5209
ORF frame 3 4710 - 5354
pBluescriptKS_primer 5228 - 5212
cPPT 5282 - 5297
U3PPT 5282 - 5303
H1_primer 5515 - 5534
loxP 5685 - 5718
HIV-1_5_LTR 5738 - 5918
truncHIV-1_3_LTR 5738 - 5918
BGH_rev_primer 5959 - 5942
bGH_PA_terminator 5945 - 6172
f1_origin 6235 - 6541
pBABE_3_primer 6675 - 6655
SV40_enhancer 6876 - 6661
SV40_promoter 6673 - 6941
SV40_origin 6840 - 6917
SV40pro_F_primer 6902 - 6921
EM7_promoter 7035 - 7102
bleo 7103 - 7474
sh_ble 7103 - 7477
SV40_PA_terminator 7610 - 7729
EBV_rev_primer 7698 - 7717
M13_reverse_primer 7791 - 7773
M13_pUC_rev_primer 7812 - 7790
lac_promoter 7855 - 7826
pBR322_origin 8783 - 8164
ORF frame 1 9798 - 8938
Ampicillin 9798 - 8938
AmpR_promoter 9868 - 9840
Unique restriction sites

NdeI 487
AgeI 3847
KpnI 3848
EcoRV 4604
XhoI 5224
BstBI 5378
ApaI 5420
MscI 7106
FspI 9233

Article: A versatile tool for conditional gene expression and knockdown. Szulc J et al. (Nat Methods. 2006 Feb . 3(2):109-16. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11653" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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