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Browse > Patrick Aebischer > Szulc et al > pLVUHshp53-tTR-KRAB
Price: $65.00
 

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TP53 plasmids
Patrick Aebischer Lab Plasmids
Didier Trono Lab Plasmids
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NCBI: TP53
TP53 antibodies
 

This is commonly
requested with
pLVUT-tTR-KRAB
pMD2.G
pLVUHshp53
pMKO.1 puro p53 shRNA 1
psPAX2
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Plasmid 11654: pLVUHshp53-tTR-KRAB
Gene/insert name: hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO
Alternative names: shRNA against p53
p53
Insert size (bp): Unknown
Gene/insert aliases: TP53, p53, LFS1, TRP53, FLJ92943
Species of gene(s): H. sapiens (human)
Vector backbone: pLVU-tTR-KRAB
(Search Vector Database)
Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp): 11579
5' Sequencing primer: See map  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: No
Please specify bacterial strain for growth and growth condition: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Sequence:View sequence
Author's Map:View map
Plasmid Provided In:Stbl3
Principal Investigator:Patrick Aebischer
Terms and Licenses:MTA

Comments: Please visit Trono lab website (http://tronolab.epfl.ch) to see frequently asked questions on cloning strategies and packaging. You may also visit LentiWeb (http://www.lentiweb.com) for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
CAG_enhancer 318 - 605
CMV_immearly_promoter 239 - 815
CMV_fwd_primer 772 - 792
HIV-1_5_LTR 835 - 1015
truncHIV-1_3_LTR 835 - 1015
HIV-1_psi_pack 1126 - 1170
RRE 1680 - 1913
ORF frame 1 1558 - 2445
cPPT 2444 - 2459
hUbC_promoter 2617 - 3833
EGFP_N_primer 3926 - 3905
EGFP 3860 - 4576
ORF frame 2 3860 - 4579
EGFP_C_primer 4513 - 4534
IRES 4624 - 5127
TetR 5205 - 5804
ORF frame 3 5190 - 6197
WPRE 6270 - 6857
ORF frame 1 6358 - 7002
pBluescriptKS_primer 6876 - 6860
cPPT 6930 - 6945
U3PPT 6930 - 6951
H1_primer 7163 - 7182
loxP 7333 - 7366
HIV-1_5_LTR 7386 - 7566
truncHIV-1_3_LTR 7386 - 7566
BGH_rev_primer 7607 - 7590
bGH_PA_terminator 7593 - 7820
f1_origin 7883 - 8189
pBABE_3_primer 8323 - 8303
SV40_enhancer 8524 - 8309
SV40_promoter 8321 - 8589
SV40_origin 8488 - 8565
SV40pro_F_primer 8550 - 8569
EM7_promoter 8683 - 8750
bleo 8751 - 9122
sh_ble 8751 - 9125
SV40_PA_terminator 9258 - 9377
EBV_rev_primer 9346 - 9365
M13_reverse_primer 9439 - 9421
M13_pUC_rev_primer 9460 - 9438
lac_promoter 9503 - 9474
pBR322_origin 10431 - 9812
ORF frame 2 11446 - 10586
Ampicillin 11446 - 10586
AmpR_promoter 11516 - 11488
Unique restriction sites

AgeI 3847
EcoRV 6252
XhoI 6872
MscI 8754
FspI 10881

Article: A versatile tool for conditional gene expression and knockdown. Szulc J et al. (Nat Methods. 2006 Feb . 3(2):109-16. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11654" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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