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Browse > Tony Pawson > Colwill et al > V622 pGEX-2TK-LP - Acceptor
Plasmid 11716: V622 pGEX-2TK-LP - Acceptor
Gene/insert name: None
Insert size (bp): Unknown
Fusion proteins or tags: GST
Terminal: N terminal on backbone
Vector backbone: NA
(Search Vector Database)
Type of vector: Bacterial expression,Cre/Lox,Acceptor vector
Backbone size (bp): 5126
5' Sequencing primer: pGEX3  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Sequence:View sequence
Author's Map:View map
Plasmid Provided In:DH5a
Principal Investigator:Tony Pawson
Terms and Licenses:MTA

Comments: Acceptor vector for the Creator Splice system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
tac_promoter 184 - 212
M13_pUC_rev_primer 224 - 246
ORF frame 3 258 - 992
GST 258 - 1134
pGEX_5_primer 869 - 891
loxP 957 - 990
AmpR_promoter 1052 - 1024
pGEX_3_primer 1198 - 1176
AmpR_promoter 1464 - 1492
ORF frame 1 1534 - 2394
Ampicillin 1534 - 2394
pBR322_origin 2549 - 3168
lacI 3466 - 4557
ORF frame 1 3598 - 4557
lac_promoter 4606 - 4635
M13_pUC_rev_primer 4649 - 4671
M13_reverse_primer 4670 - 4688
M13_forward20_primer 4716 - 4700
M13_pUC_fwd_primer 4731 - 4709
lacZ_a 4697 - 4852
Unique restriction sites

MscI 465
BstBI 655
BamHI 951
EcoRI 1118
AatII 1402
PstI 2079
ApaI 4036
EcoRV 4275
HpaI 4331
NarI 4465

Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11716" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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