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Browse > Tony Pawson > Colwill et al > V1579 pLP ProHis HTb - Acceptor
Plasmid 11718: V1579 pLP ProHis HTb - Acceptor
Gene/insert name: None
Insert size (bp): Unknown
Fusion proteins or tags: His
Terminal: N terminal on backbone
Vector backbone: NA
(Search Vector Database)
Type of vector: Bacterial expression,Cre/Lox,Acceptor vector
Backbone size (bp): 4939
5' Sequencing primer: pBAD_rev  (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Sequence:View sequence
Author's Map:View map
Plasmid Provided In:DH5a
Principal Investigator:Tony Pawson
Terms and Licenses:MTA

Comments: Acceptor vector for the Creator Splice system.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features
trc_promoter 193 - 266
M13_pUC_rev_primer 235 - 257
loxP 351 - 384
AmpR_promoter 446 - 418
pBAD_rev_primer 650 - 633
pTrcHis_rev_primer 650 - 633
rrnB_terminator 683 - 840
rrnB_T1_terminator 806 - 849
rrnB_T2_terminator 981 - 1008
AmpR_promoter 1050 - 1078
ORF frame 1 1120 - 1980
Ampicillin 1120 - 1980
pBR322_origin 2135 - 2754
f1_origin 3398 - 3092
pGEX_3_primer 3640 - 3662
lacI 3809 - 4900
ORF frame 2 3941 - 4900
Unique restriction sites

NarI 337
NcoI 340
BamHI 345
EcoRI 512
StuI 522
SalI 528
SacI 538
SpeI 540
NotI 548
EagI 548
BstBI 557
XbaI 562
PstI 574
XhoI 577
KpnI 593
HindIII 595
FspI 1684
BclI 4182
ApaI 4379
HpaI 4674

Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11718" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.

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