Addgene - pL3-TRE-MCS-polyA-2L Plasmid Data

Browse > Geoff Wahl > Wong et al > pL3-TRE-MCS-polyA-2L

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Geoff Wahl Lab Plasmids

This is commonly
requested with

RV-L3-HyTK-2L

pL3-TRE-LucGFP- 2L

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Plasmid 11719: pL3-TRE-MCS-polyA-2L

Gene/insert name:		None
Insert size (bp):		Unknown
Vector backbone:		None (Search Vector Database)
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		4706
5' Sequencing primer:		See map (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		TetO sites, MCS, and polyA was cloned from pTRE2 (BD Bioscience) into the backbone.
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Geoff Wahl
Terms and Licenses:		MTA, Tet Systems LULL

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

pGEX_3_primer

51 - 29

lacZ_a

398 - 250

M13_pUC_fwd_primer

364 - 386

M13_forward20_primer

379 - 395

M13_forward20_primer

428 - 444

loxP

510 - 477

loxP

477 - 510

TRE

527 - 844

pCEP_fwd_primer

877 - 896

LNCX_primer

879 - 903

CMV2_promoter

846 - 965

bGlob_int

1080 - 1652

rb_glob_PA_terminator

1704 - 2217

loxP

2418 - 2451

M13_reverse_primer

2499 - 2481

M13_pUC_rev_primer

2520 - 2498

lac_promoter

2563 - 2534

pBR322_origin

3491 - 2872

ORF frame 1

4506 - 3646

Ampicillin

4506 - 3646

AmpR_promoter

4576 - 4548

Unique restriction sites

NarI		236
XhoI		527
StuI		865
SacII		969
BamHI		997
NheI		1021
NotI		1028
EcoRV		1055
NcoI		1196
ApaI		1611
MscI		1752
BglII		1781
PstI		2459
AatII		4641

Article: Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Wong ET et al. (Nucleic Acids Res. 2005 . 33(17):e147. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11719" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.