Addgene - pGL3-p300 reporter Plasmid Data

Gene/insert name:		p300 promoter and 5' UTR
Alternative names:		p300
Insert size (bp):		1533
Gene/insert aliases:		EP300, p300, KAT3B
Fusion proteins or tags:		Luciferase
Terminal:		C terminal on backbone
Vector backbone:		pGL3-Basic (Search Vector Database)
Backbone manufacturer:		Promega
Type of vector:		Mammalian expression,Luciferase
Backbone size (bp):		4818
Cloning site 5':		KpnI
Site destroyed during cloning:		Unknown
Cloning site 3':		HindIII
Site destroyed during cloning:		Unknown
5' Sequencing primer:		RVprimer3 (List of Sequencing Primers)
3' Sequencing primer:		LucNrev
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Eileen Adamson
Terms and Licenses:		MTA, Luciferase LULL

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Coactivating factors p300 and CBP are transcriptionally crossregulated by Egr1 in prostate cells, leading to divergent responses. Yu J et al. (Mol Cell. 2004 Jul 2. 15(1):83-94. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11726" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.