Addgene - pcDNA3-Egr1 Plasmid Data

Gene/insert name:		Egr-1
Insert size (bp):		Unknown
Gene/insert aliases:		Egr1, egr, TIS8, Zenk, Egr-1, NGFIA, Zfp-6, ETR103, Krox-1, Krox24, NGF1-A, NGFI-A, Zif268, Krox-24, A530045N19Rik
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		FLAG
Terminal:		N terminal on insert
Vector backbone:		pcDNA3 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		5400
Cloning site 5':		EcoRV
Site destroyed during cloning:		Unknown
Cloning site 3':		EcoRV
Site destroyed during cloning:		Unknown
5' Sequencing primer:		T7 (List of Sequencing Primers)
3' Sequencing primer:		Sp6
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Eileen Adamson
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Coactivating factors p300 and CBP are transcriptionally crossregulated by Egr1 in prostate cells, leading to divergent responses. Yu J et al. (Mol Cell. 2004 Jul 2. 15(1):83-94. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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