Addgene - pCXN2-mVIN Plasmid Data

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Plasmid 11776: pCXN2-mVIN

Gene/insert name:		vinculin
Insert size (bp):		3200
Gene/insert aliases:		Vcl, AA571387, AI462105, AW545629, 9430097D22
Species of gene(s):		M. musculus (mouse)
Vector backbone:		pCXN2 (Search Vector Database)
Backbone manufacturer:		Niwa et al, Gene, v108, 1991
Type of vector:		Mammalian expression
Backbone size (bp):		5200
Cloning site 5':		See author map
Site destroyed during cloning:		Unknown
Cloning site 3':		See author map
Site destroyed during cloning:		Unknown
5' Sequencing primer:		See author map (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Neomycin
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Eileen Adamson
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Rescue of the mutant phenotype by reexpression of full-length vinculin in null F9 cells; effects on cell locomotion by domain deleted vinculin. Xu W et al. (J Cell Sci. 1998 Jun . 111 ( Pt 11)():1535-44. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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