Addgene - pET-NEMO Plasmid Data

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NM_003639

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Plasmid 11966: pET-NEMO

Gene/insert name:		NEMO
Alternative names:		IKKgamma
Insert size (bp):		1290
GenBank/Entrez ID of insert:		NM_003639
Gene/insert aliases:		IKBKG, IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma
Species of gene(s):		H. sapiens (human)
Fusion proteins or tags:		His
Terminal:		N terminal on backbone
Vector backbone:		pET28a (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		5370
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Jon Ashwell
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ et al. (Nat Cell Biol. 2006 Apr . 8(4):398-406. Pubmed)

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