Addgene - pCMVTAG-NEMOL329P Plasmid Data

Gene/insert name:		NEMOL329P
Alternative names:		NEMO
		IKKgamma
Insert size (bp):		1290
GenBank/Entrez ID of insert:		NM_003639
Gene/insert aliases:		IKBKG, IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, IKK-gamma
Species of gene(s):		H. sapiens (human)
Relevant mutations/deletions:		Leucine329 to Proline
Fusion proteins or tags:		Flag
Terminal:		N terminal on backbone
Vector backbone:		pCMVTAG2B (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4300
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		T3 (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Jon Ashwell
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ et al. (Nat Cell Biol. 2006 Apr . 8(4):398-406. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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