Addgene - pGEX-RIP Plasmid Data

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NM_009068

NCBI: Ripk1

Ripk1 antibodies

This is commonly
requested with

HA-CYLD-WT

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Plasmid 11972: pGEX-RIP

Gene/insert name:		RIP
Alternative names:		RIP1
Insert size (bp):		1950
GenBank/Entrez ID of insert:		NM_009068
Gene/insert aliases:		Ripk1, RIP, Rinp, Rip1, D330015H01Rik
Species of gene(s):		M. musculus (mouse)
Fusion proteins or tags:		GST
Terminal:		N terminal on backbone
Vector backbone:		pGEX4T1 (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		4900
Cloning site 5':		BamHI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		pGEX 5' primer (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Jon Ashwell
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: NEMO is a sensor of Lys 63-linked polyubiquitination and functions in NF-kappaB activation. Wu CJ et al. (Nat Cell Biol. 2006 Apr . 8(4):398-406. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 11972" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.