Addgene - DH10beta F' DOT sbcC (donor strain) Plasmid Data

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Stephen Elledge Lab Plasmids

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BUN21/pML300 (recipient strain)

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Product 12082: DH10beta F' DOT sbcC (donor strain)

Gene/insert name:		F' Tra operon
Insert size (bp):		Unknown
Vector backbone:		na (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		Unknown
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Tetracycline
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		Yes
Type of Material:		Bacterial Strain
Principal Investigator:		Stephen Elledge
Additional Documents:		Elledge-pPRIME (PDF)
Terms and Licenses:		MTA

Comments: F' contains a transfer operon (tra) and oriT is replaced with the tetracycline-resistant gene (tetR). This modified F' can mobilize the donor plasmid but not itself. The bacterial strain is DH10beta F' DOT sbcC (donor host strain): mcrA delta(mrr-hsdRMS-mcrBC) phi80lacZdeltaM15 delta-lacX74 deoR recA1 endA1 ara-delta139 delta(ara, leu)7697 galU galK lambda- rpsL nupG tonA umuC::pir116-frt F'(lac+ pro+ delta-oriT::Tc) sbcC::Frt.

Article: A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Stegmeier F et al. (Proc Natl Acad Sci U S A. 2005 Sep 13. 102(37):13212-7. Pubmed)

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