Addgene - pSico PGK GFP Plasmid Data

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Plasmid 12093: pSico PGK GFP

Gene/insert name:		none
Insert size (bp):		Unknown
Relevant mutations/deletions:		EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. Cre causes EGFP to be recombined out of the construct, activating shRNA expression.
Vector backbone:		pSico PGK GFP (Search Vector Database)
Type of vector:		Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp):		7500
Cloning site 5':		HpaI
Site destroyed during cloning:		No
Cloning site 3':		XhoI
Site destroyed during cloning:		No
5' Sequencing primer:		EGFP-C (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Tyler Jacks
Terms and Licenses:		MTA

Comments: pSico PGK GFP is similar to pSico, except the CMV promoter is replaced with the PGK promoter. pSico PGK GFP allows for conditional (Cre-Lox), stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS ON shRNA expression.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author's map or on the Jacks Lab website (http://web.mit.edu/ccr/labs/jacks/protocols/pSico.html).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Cre-lox-regulated conditional RNA interference from transgenes. Ventura A et al. (Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12093" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.