Addgene - HA-Foxo1ADA (pCMV5) Plasmid Data

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This is commonly
requested with

HA-Foxo1 (pCMV5)

Myc-Foxo1 D256

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Plasmid 12143: HA-Foxo1ADA (pCMV5)

Gene/insert name:		Foxo1
Alternative names:		Fkhr
Insert size (bp):		2100
Gene/insert aliases:		Foxo1, Afxh, FKHR, Fkhr1, Foxo1a, AI876417
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		Phosphorylation-deficient, also referred to as constitutivley active. Contians three point mutaitons: Thr24Ala, Ser253Asp, Ser316Ala
Fusion proteins or tags:		HA
Terminal:		N terminal on backbone
Vector backbone:		pCMV5 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4657
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		XbaI
Site destroyed during cloning:		No
5' Sequencing primer:		CMV-F (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Domenico Accili
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: The forkhead transcription factor Foxo1 (Fkhr) confers insulin sensitivity onto glucose-6-phosphatase expression. Nakae J et al. (J Clin Invest. 2001 Nov . 108(9):1359-67. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12143" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.