Addgene - Myc-Foxo3 (pCMV5) Plasmid Data

Browse > Nakae et al > Myc-Foxo3 (pCMV5)

Unavailable

Help: Plasmid Unavailable
This plasmid is currently not available for distribution from Addgene. Please check back later. If you have further questions, please contact Addgene.

Plasmid Links

Related Plasmids

Other Links

This is commonly
requested with

FLAG-FOXO3a WT

FLAG-Foxo1( pCMV5)

Print Friendly

Plasmid 12144: Myc-Foxo3 (pCMV5)

Gene/insert name:		Foxo3
Alternative names:		Fkrh
Insert size (bp):		2100
GenBank/Entrez ID of insert:		NM_019739
Gene/insert aliases:		Foxo3, Fkhr2, C76856, FKHRL1, Foxo3a, 1110048B16Rik, 2010203A17Rik
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		Wild-type
Fusion proteins or tags:		Myc
Terminal:		N terminal on insert
Vector backbone:		pCMV5 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4657
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		XbaI
Site destroyed during cloning:		No
5' Sequencing primer:		CMV-F (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Domenico Accili
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: The forkhead transcription factor Foxo1 (Fkhr) confers insulin sensitivity onto glucose-6-phosphatase expression. Nakae J et al. (J Clin Invest. 2001 Nov . 108(9):1359-67. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12144" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.