Addgene - Igfbp1-promoter/pGL3 Plasmid Data

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Igfbp1 antibodies

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Plasmid 12146: Igfbp1-promoter/pGL3

Gene/insert name:		IGFBP1 promoter
Alternative names:		Igfbp1
Insert size (bp):		Unknown
Gene/insert aliases:		Igfbp1, IBP1, IGFBA, IGF-BP25
Species of gene(s):		R. norvegicus (rat)
Relevant mutations/deletions:		Contains the canonical insulin response sequence (IRS) from the rat IGF binding protein-1 promoter driving a luciferase reporter
Fusion proteins or tags:		Luciferase
Terminal:		C terminal on backbone
Vector backbone:		pGL3 basic (Search Vector Database)
Backbone manufacturer:		Promega
Type of vector:		Mammalian expression
Backbone size (bp):		4818
5' Sequencing primer:		N/A (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Domenico Accili
Terms and Licenses:		MTA, Luciferase LULL

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Differential regulation of gene expression by insulin and IGF-1 receptors correlates with phosphorylation of a single amino acid residue in the forkhead transcription factor FKHR. Nakae J et al. (EMBO J. 2000 Mar 1. 19(5):989-96. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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