Addgene - FLAG-Foxo1(pCMV5) Plasmid Data

Gene/insert name:		Foxo1
Alternative names:		Fkhr
Insert size (bp):		2000
GenBank/Entrez ID of insert:		NM_019739
Gene/insert aliases:		Foxo1, Afxh, FKHR, Fkhr1, Foxo1a, AI876417
Species of gene(s):		M. musculus (mouse)
Relevant mutations/deletions:		NON
Fusion proteins or tags:		FLAG
Terminal:		N terminal on insert
Vector backbone:		pCMV5 (Search Vector Database)
Type of vector:		Mammalian expression
Backbone size (bp):		4700
Cloning site 5':		KpnI
Site destroyed during cloning:		No
Cloning site 3':		XbaI
Site destroyed during cloning:		No
5' Sequencing primer:		CGTTGACGCAAATGGGCGG (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Domenico Accili
Terms and Licenses:		MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: FoxO1 protects against pancreatic beta cell failure through NeuroD and MafA induction. Kitamura YI et al. (Cell Metab. 2005 Sep . 2(3):153-63. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12148" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.