Addgene - pCS Plasmid Data

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Inder Verma Lab Plasmids

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Plasmid 12158: pCS

Gene/insert name:		none
Insert size (bp):		Unknown
Vector backbone:		pCS (Search Vector Database)
Backbone manufacturer:		Verma Lab
Type of vector:		Mammalian expression,Lentiviral
Backbone size (bp):		7140
5' Sequencing primer:		n/a (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		Stbl3
Principal Investigator:		Inder M Verma
Terms and Licenses:		MTA

Comments: pCS has a 133-bp deletion in the 3' LTR, generating a self-inactivating (SIN) virus. See article for cloning details.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

CAG_enhancer

315 - 602

CMV_immearly_promoter

236 - 812

CMV_fwd_primer

769 - 789

HIV-1_5_LTR

832 - 1012

truncHIV-1_3_LTR

832 - 1012

HIV-1_psi_pack

1120 - 1164

RRE

1674 - 1907

ORF frame 1

1552 - 2967

cPPT

2604 - 2619

U3PPT

2604 - 2625

HIV-1_5_LTR

2941 - 3121

truncHIV-1_3_LTR

2941 - 3121

BGH_rev_primer

3164 - 3147

bGH_PA_terminator

3150 - 3377

f1_origin

3440 - 3746

pBABE_3_primer

3880 - 3860

SV40_enhancer

4082 - 3866

SV40_promoter

3878 - 4147

SV40_origin

4046 - 4123

SV40pro_F_primer

4108 - 4127

EM7_promoter

4241 - 4308

bleo

4309 - 4680

sh_ble

4309 - 4683

SV40_PA_terminator

4816 - 4935

EBV_rev_primer

4904 - 4923

M13_reverse_primer

4997 - 4979

M13_pUC_rev_primer

5018 - 4996

lac_promoter

5061 - 5032

pBR322_origin

5989 - 5370

ORF frame 3

7004 - 6144

Ampicillin

7004 - 6144

AmpR_promoter

7074 - 7046

Unique restriction sites

SpeI		249
NdeI		484
NarI		1016
NotI		1519
BamHI		2380
SacII		2398
PstI		2408
EcoRI		2409
ClaI		2416
XbaI		2421
HpaI		2429
XhoI		2432
KpnI		2554
ApaI		3126
StuI		4179
MscI		4312
FspI		6439

Article: Development of a self-inactivating lentivirus vector. Miyoshi H et al. (J Virol. 1998 Oct . 72(10):8150-7. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12158" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.