Addgene - Pdx-PH2/pGL3 Plasmid Data

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Domenico Accili Lab Plasmids

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AF334615

This is commonly
requested with

GADD45 WT-luc

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Plasmid 12163: Pdx-PH2/pGL3

Gene/insert name:		Pdx1 promoter PH2 domain
Insert size (bp):		138
GenBank/Entrez ID of insert:		AF334615
Species of gene(s):		M. musculus (mouse)
Vector backbone:		pGL3 (Search Vector Database)
Type of vector:		Luciferase
Backbone size (bp):		4818
Cloning site 5':		XhoI
Site destroyed during cloning:		No
Cloning site 3':		HindIII
Site destroyed during cloning:		No
5' Sequencing primer:		See pGL3 vector (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Unknown
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		Domenico Accili
Terms and Licenses:		MTA, Luciferase LULL

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: The forkhead transcription factor Foxo1 links insulin signaling to Pdx1 regulation of pancreatic beta cell growth. Kitamura T et al. (J Clin Invest. 2002 Dec . 110(12):1839-47. Pubmed)

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