Addgene - pMB80 (R26-CreER) Plasmid Data

Browse > Tyler Jacks > McLaughlin et al > pMB80 (R26-CreER)

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Hsp68-CreER( TM) (DM#264)

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Plasmid 12168: pMB80 (R26-CreER)

Gene/insert name:		Rosa26 CreER^T2
Alternative names:		CreER
Insert size (bp):		Unknown
Vector backbone:		pROSA26-1 (Search Vector Database)
Backbone manufacturer:		P. Soriano
Type of vector:		Mammalian expression,Cre/Lox
Backbone size (bp):		Unknown
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		XbaI
Site destroyed during cloning:		No
5' Sequencing primer:		na (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Selectable markers:		Puromycin
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid:		CreER^T2 cDNA from P. Chambon (Indra et al., 1999)
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Tyler Jacks
Terms and Licenses:		MTA

Comments: Targeting vector to generate R26-CreER^T2 mice. XbaI/MluI SA-CreER^T2 fragment of pMB61, MluI/XbaI PA-floxed PGK-Puro fragment of pMB75, and XbaI-digested modified version of pROSA26-1 vector where SacII site is replaced by an AscI linearization site.

See sequence link for sequence of insert from author. Please note that there are minor errors in the sequence, specifically there are no XbaI sites within the insert. Please see Addgene test digest with XbaI for the expected bands.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Article: The Nf2 tumor suppressor regulates cell-cell adhesion during tissue fusion. McLaughlin ME et al. (Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3261-6. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12168" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.