Addgene - pSty11 Plasmid Data

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Titia de Lange Lab Plasmids

This is commonly
requested with

pSXneo 135( T2AG3)

pSXneo 270( T2AG3)

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Plasmid 12401: pSty11

Gene/insert name:		Sty11
Alternative names:		800bp T2AG3 repeat
		21
Insert size (bp):		800
Vector backbone:		pSP73 (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		2400
Cloning site 5':		EcoRI
Site destroyed during cloning:		No
Cloning site 3':		EcoRI
Site destroyed during cloning:		No
5' Sequencing primer:		T7 (List of Sequencing Primers)
3' Sequencing primer:		SP6
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Titia de Lange
Terms and Licenses:		MTA

Comments: Insert used as probe for telomere blots

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: Human telomeres are attached to the nuclear matrix. de Lange T et al. (EMBO J. 1992 Feb . 11(2):717-24. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12401" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.