Addgene - CFr Plasmid Data

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David Baker Lab Plasmids

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Plasmid 12482: CFr

Gene/insert name:		CFr
Insert size (bp):		189
Species of gene(s):		Other
Fusion proteins or tags:		6xHis
Terminal:		N terminal on insert
Vector backbone:		pet29 b(+) (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		5371
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Please specify bacterial strain for growth and growth condition:		Plasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		David Baker
Terms and Licenses:		MTA

This plasmid has not been sequence verified. If you would like Addgene to sequence a portion of this plasmid before you request it, please contact us.

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