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Plasmid 12499: FKB

Gene/insert name:		FKB
Alternative names:		FKBP12
Insert size (bp):		417
Species of gene(s):		Other
Relevant mutations/deletions:		RosettaDesign was used to design sequences. Alignment to wild-type can be seen in the article.
Fusion proteins or tags:		6xHis
Terminal:		C terminal on insert
Vector backbone:		pet29 b(+) (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		5371
5' Sequencing primer:		T7 (List of Sequencing Primers)
Bacteria resistance:		Kanamycin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Please specify bacterial strain for growth and growth condition:		Plasmids are provided in DH5 alpha, but for protein expression, BL21(DE3)pLysS is recommended.
Sequence:		View sequence
Plasmid Provided In:		DH5a
Principal Investigator:		David Baker
Terms and Licenses:		MTA

Comments: A synthetic gene which placed the sequence under the control of the T7 promoter, a C-terminal 6X His tag, and codon usage optimized for Escherichia coli was obtained from BlueHeron Biotechnologies. The gene was then subcloned into the pet29 b(+) vector from Novagen.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Article: A large scale test of computational protein design: folding and stability of nine completely redesigned globular proteins. Dantas G et al. (J Mol Biol. 2003 Sep 12. 332(2):449-60. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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