Article: Increased efficiency of cloning large DNA fragments using a lower copy number plasmid. Feng T et al. (Biotechniques. 2002 May . 32(5):992, 994, 996 passim. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12514" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.