Addgene - US0hisB-pyrF- Plasmid Data

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Product 12614: US0hisB-pyrF-

Gene/insert name:		Bacteria hybrid selection strain
Insert size (bp):		Unknown
Vector backbone:		XL-1 blue F' episome (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		Unknown
5' Sequencing primer:		N/A (List of Sequencing Primers)
Bacteria resistance:		Tetracycline
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		Yes
Type of Material:		Bacterial Strain
Principal Investigator:		Scot Wolfe
Terms and Licenses:		MTA

Comments: This strain has a deletion in both the hisB and pyrF genes (the bacterial homologs of HIS3 and URA3) and it contains a F' episome bearing the lacl^q repressor.

Article: A bacterial one-hybrid system for determining the DNA-binding specificity of transcription factors. Meng X et al. (Nat Biotechnol. 2005 Aug . 23(8):988-94. Pubmed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.

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